We narrowed to 2,230 results for: Kap

1. pCMV4-HA-NEMO

   Plasmid

   #27560

   Depositor

   Shao-Cong Sun

   Article

   Harhaj et al J Biol Chem. 1999 Aug 13. 274(33):22911-

   Insert

   NEMO (IKBKG Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S8R

   Promoter

   Available since

   Aug. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)

   Plasmid

   #174639

   Purpose

   Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-GFP-ppKinVIb(861-1321)
   

   Use

   Tags

   SSPB(micro)-GFP

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…

   Promoter

   Chicken beta-actin

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK597

   Plasmid

   #71121

   Purpose

   Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

   Depositor

   T. J. Kappock

   Article

   Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

   Insert

   formyl-CoA:oxalate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV6-AN-HA_aa 1-542 POLK

   Plasmid

   #201585

   Purpose

   Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   Polymerase kappa (POLK Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Expresses amino acids 1-542 of DNA polymerase kap…

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

   Plasmid

   #205603

   Purpose

   Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

   Depositor

   Tarun Kapoor

   Article

   Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

   Insert

   γ-tubulinWT-RNAi-resistant-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G2228A, C2231T, C2237T, G2240A

   Promoter

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

   Plasmid

   #205604

   Purpose

   Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

   Depositor

   Tarun Kapoor

   Article

   Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

   Insert

   γ-tubulinΔGTP-RNAi-resistant-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G2228A, C2231T, C2237T, G2240A, N229A

   Promoter

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)

   Plasmid

   #174641

   Purpose

   Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   SSPB(micro)-mVenus-ppKin14VIb(861-1321)
   

   Use

   Tags

   SSPB(micro)-Venus

   Expression

   Mammalian

   Mutation

   SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

   Promoter

   Chicken beta-actin

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pACEBac1-gamma-tubulin-TEV-His6

   Plasmid

   #178067

   Purpose

   Insect cell expression of gamma-tubulin-TEV-His6

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

   Insert

   gamma-tubulin (TUBG1 Human)
   

   Use

   Tags

   TEV-His6

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNC2

   Plasmid

   #72438

   Purpose

   Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)

   Depositor

   T. J. Kappock, JoAnne Stubbe

   Article

   Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Pro64 to Arg; Asp65 to His; Gln206 to Arg

   Promoter

   T7 and n/a

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK516

    Plasmid

    #71578

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-435 to glutamine

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK524

    Plasmid

    #71580

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes asparagine-347 to alanine

    Promoter

    T7

    Available since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK513

    Plasmid

    #71576

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-294 to alanine

    Promoter

    T7

    Available since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK564

    Plasmid

    #71581

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes serine-71 to alanine

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK517

    Plasmid

    #71579

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-435 to aspartate

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK514

    Plasmid

    #71577

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamine-435 to alanine

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK512

    Plasmid

    #71575

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes arginine-228 to glutamate

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK048

    Plasmid

    #75230

    Purpose

    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

    Depositor

    T. J. Kappock, JoAnne Stubbe

    Article

    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

    Promoter

    T7 and n/a

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. polyQ74-GFP

    Plasmid

    #231893

    Purpose

    Forms cytosolic HTT partial exon 1 Q74 aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA4.TO-K5-2xCSTREP

    Plasmid

    #136154

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K5

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    K5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pB80-hKif5b(1-560)rigor-2xmNeongreen

    Plasmid

    #174649

    Purpose

    Live-cell marker for stable microtubules

    Depositor

    Lukas Kapitein

    Article

    Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

    Insert

    hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
    

    Use

    Tags

    mNeongreen-mNeongreen

    Expression

    Mammalian

    Mutation

    rigor mutation G234A

    Promoter

    Chicken beta-actin

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge