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Plasmid#227358PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin R104A (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin Ubl P37L
Plasmid#227366PurposeFor bacterial expression of mutated parkin Ubl.DepositorInsertParkin 1-76 P37L (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin D106A
Plasmid#227362PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin D106A (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin V105K
Plasmid#227361PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin V105K (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin T103A
Plasmid#227360PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin T103A (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin L102K
Plasmid#227359PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin L102K (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin M458L
Plasmid#227356PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin M458L (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin R256C
Plasmid#227355PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin R256C (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin R234Q
Plasmid#227354PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin R234Q (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin V224A
Plasmid#227353PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin V224A (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin P37L
Plasmid#227352PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin P37L (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin delta 101-109
Plasmid#227364PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin delta 101-109 (PRKN Human)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Resi…PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPP5912
Plasmid#128703PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcE-avrE1
UseTagsExpressionBacterialMutationThree synonymous mutations Ala 362 (GCT-GCC), Ala…PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
aL-EGFP
Plasmid#103879Purposefull-length human aL with EGFP at betapropellerDepositorInsertfull-length human aL with EGFP in betapropeller (ITGAL Human)
UseTagstension sensor moduleExpressionMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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aL-L3
Plasmid#103881Purposefull-length human aL with moxsynGFP in betapropellerDepositorInsertfull-length human aL with moxsynGFP in betapropeller (ITGAL Human)
UseTagstension sensor moduleExpressionMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
ET8-GPIba-high-6His
Plasmid#180251PurposeHigh affinity mutant of human platelet membrane protein GP1b alphaDepositorInserthuman GPIbalpha 1-290aa with mutation G233V and M239V (GP1BA Human)
UseTags6HisExpressionMammalianMutationG233V and M239VPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA blast
Plasmid#104993PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA puro
Plasmid#104990PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only