We narrowed to 1,587 results for: Tef

1. JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)

   Plasmid

   #156410

   Purpose

   pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12V

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12V (KRAS Human)
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   mutant KRAS4A-G12V

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)

   Plasmid

   #156409

   Purpose

   pCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12D

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12D (KRAS Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   mutant KRAS4A-G12D

   Promoter

   CMV

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

   Plasmid

   #156415

   Purpose

   kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12D (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   mTagBFP2

   Expression

   Mutation

   mutant KRAS4A-G12D

   Promoter

   kdrl

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)

   Plasmid

   #156416

   Purpose

   kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12V (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   mScarlet-I

   Expression

   Mutation

   mutant KRAS4A-G12V

   Promoter

   kdrl

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. KRAS-A146T

   Plasmid

   #154426

   Purpose

   A146T Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

   Depositor

   Kenneth Westover

   Article

   Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

   Insert

   KRAS (KRAS Human)
   

   Use

   Tags

   6xHis-tag and TEV protease cleavage sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)

   Plasmid

   #180468

   Purpose

   kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   KRAS4A (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   V5-mScarlet-I

   Expression

   Mutation

   Promoter

   kdrl

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)

   Plasmid

   #156406

   Purpose

   Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4A-G12V (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   mScarlet-I

   Expression

   Mutation

   mutant KRAS4A-G12V

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

   Plasmid

   #156414

   Purpose

   kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4B-G12V (KRAS Human)
   

   Use

   Zebrafish transgenesis

   Tags

   EGFP

   Expression

   Mutation

   mutant KRAS4B-G12V

   Promoter

   kdrl

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGGG_Sr1644-1Sh

   Plasmid

   #164087

   Purpose

   Expresses pGGG_Sr1644-1Sh in plants

   Depositor

   Brande Wulff

   Article

   Yu et al Nat Commun. 2022 Mar 25;13(1):1607. doi:

   Insert

   pGGG_Sr-1644-1Sh
   

   Use

   Tags

   none

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Native promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pαH-rS2d

    Plasmid

    #183516

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein S-GSAS-rS2d with (682-685) furin side replaced with GSAS and mutation S383C, D985C

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    rS2d (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)

    Plasmid

    #156413

    Purpose

    kdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4B-WT (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    kdrl

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

    Plasmid

    #156404

    Purpose

    Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    mTagBFP2

    Expression

    Mutation

    mutant KRAS4A-G12D

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

    Plasmid

    #213024

    Purpose

    Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

    Depositor

    Oliver Weichenrieder, Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (238-1061)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7 / Lac

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3EF1aH-myr-Akt

    Plasmid

    #179909

    Purpose

    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

    Depositor

    Xin Chen

    Article

    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pαH-rS2d-HexaPro

    Plasmid

    #183515

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pαH-S-GSAS-OMICRON.BA.5

    Plasmid

    #213069

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    S-GSAS-Omicron.BA5 (S )
    

    Use

    Tags

    HRV 3C cleavage site (before tags) C terminal on …

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pαH-S-RRAR-OMICRON

    Plasmid

    #180424

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side intact

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

    Insert

    Spike (S-RRAR-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pαH-S-GSAS-OMICRON.BA.2

    Plasmid

    #184829

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

    Insert

    S-GSAS-OMICRON.BA.2 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pαH-S-RRAR/XBB.1.16

    Plasmid

    #213037

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.16 with with furin side intact

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    s -RRAR.XBB.1.16 (S )
    

    Use

    Tags

    HRV 3C cleavage site (before tags) 8X His tag 2X…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 furin site RRAR …

    Promoter

    CMV

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pαH-S-RRAR-OMICRON.BA.5

    Plasmid

    #213071

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.5 with with furin side intact

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pαH-S-RRAR-OMICRON.BA.5 (S )
    

    Use

    Tags

    HRV 3C cleavage site (before tags) C terminal on …

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685, furin site inta…

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge