We narrowed to 9,088 results for: ef1

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mc2 hygro EF1a

Plasmid

#206222

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-π-EB1_EF1α-mCherry

Plasmid

#197429

Purpose

Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)

Use

CRISPR and Synthetic Biology

Tags

GCN4 leucine zipper, LOV2, Zdk1, and mCherry

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-π-EB1_EF1α-EGFP

Plasmid

#197430

Purpose

Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)

Use

CRISPR and Synthetic Biology

Tags

EGFP, GCN4 leucine zipper, LOV2, and Zdk1

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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vYM004_pNeo_phiC31_nTRE_pEF1s(orig)_citrine

Plasmid

#197677

Purpose

"pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-F1.218.CAR-3G

Plasmid

#194460

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmeEF1A_R

Plasmid

#147347

Purpose

Insect Expression of DmeIF1A

Depositor

Elisa Izaurralde

Insert

DmeIF1A (eIF-1A Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeEF1A_R

Plasmid

#147349

Purpose

Insect Expression of DmeIF1A

Depositor

Elisa Izaurralde

Insert

DmeIF1A (eIF-1A Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-ArgSen

Plasmid

#184691

Purpose

Lentiviral transfer plasmid for expression of ArgSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-ArgSen

Plasmid

#184700

Purpose

Lentiviral transfer plasmid for expression of ArgSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-pHCountdown

Plasmid

#182301

Purpose

lentiviral plasmid encoding pH-Countdown and puromycin selection

Depositor

Andrew York

Article

Seidel et al (2021), doi: 10.5281/zenodo.5810930

Insert

pH countdown

Use

Lentiviral

Promoter

EF-1a

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α -NWM-Node 1

Plasmid

#154253

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 1 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 1 of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α -NWM-Node 2

Plasmid

#154254

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 2 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 2 of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α -NWM-Node 3

Plasmid

#154255

Purpose

expresses reconstructed New World monkey retroCHMP3 Node 3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed Node 3 of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_MAPK9-P2A-Hygro_Barcode

Plasmid

#170238

Purpose

Barcoded lentiviral vector to express MAPK9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

MAPK9 (MAPK9 Human)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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MS2592.EF1a.SLC6A1.S295L.RFP.P2A.PURO.JL.p163

Plasmid

#170174

Purpose

Lentiviral Expression of EF1a.SLC6A1.S295L.RFP

Depositor

Christopher Moxham

Insert

SLC6A1 (SLC6A1 Human)

Use

Lentiviral

Mutation

S295L

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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MS2592.EF1a.SLC6A1.A288V.P2A.PURO.JL.p166

Plasmid

#170171

Purpose

Lentiviral Expression of EF1a.SLC6A1.A288V

Depositor

Christopher Moxham

Insert

SLC6A1 (SLC6A1 Human)

Use

Lentiviral

Mutation

A288V

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-HaloSFPQY527A

Plasmid

#166949

Purpose

Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Use

Lentiviral

Tags

Halo

Mutation

SFPQ-Y527A

Promoter

eF1a

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD

Plasmid

#162811

Purpose

Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_(C1A)-Intein_DD

Use

Piggybac

Expression

Mammalian

Mutation

Intein(Cys1,Ala)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)

Plasmid

#162822

Purpose

STELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)CoV2_ORF3b(CoV1h)

Use

Piggybac

Expression

Mammalian

Mutation

CoV2_ORF3b(CoV1h)(Met1,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI

Plasmid

#162803

Purpose

Control STELLA destination plasmid for N-terminally labelled proteins

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub(G75,76A)-BsmBI

Use

Piggybac

Expression

Mammalian

Mutation

Ubiquitin (G75,76A)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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