We narrowed to 2,376 results for: pET28

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pET28 em-blue-shift.2

Plasmid

#191901

Purpose

Encodes a GFP design, em-blue-shift.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

em-blue-shift.2

Tags

His Tag

Expression

Bacterial

Mutation

T65S S72A T108I Y145F V150I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1CAN-MutTER

Plasmid

#178033

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

Changed T(108)A, E(109)A, R(110)A

Promoter

T7LacI

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-MBP-CI2F

Plasmid

#191254

Purpose

Expresses chymotrypsin inhibitor with an N-terminal MBP fusion protein

Depositor

Arthur Laganowsky

Article

Raab et al J Am Chem Soc. 2020 Oct 14;142(41):17372

Insert

Chymotrypsin Inhibitor 2

Tags

Maltose Binding Protein

Expression

Bacterial

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-matNODALvar-HIS

Plasmid

#115265

Purpose

For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL variant mature peptide (NODAL Human)

Tags

6His

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-eGFP-Pep-H

Plasmid

#185788

Purpose

Encodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

eGFP-Pep4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-MBP-bhaB5

Plasmid

#167812

Purpose

Expresses His6- and MBP-tagged BhaB5 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaB5

Tags

His6, MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

Plasmid

#183387

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

redesigned Caspase-4 (AA94-377) (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

ANR261ENP, D315H, M318K, T352K

Promoter

T7

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1ΔN (MT2B2)

Plasmid

#178034

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

First N-Terminal 27aa are deleted

Promoter

T7LacI

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-CPL2/pET28a

Plasmid

#172095

Purpose

cDNA of CER11/CPL2 cloned into pET28a for protein expression

Depositor

George Haughn

Article

Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

Insert

CPL2 (CPL2 Mustard Weed)

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET28_6xHis-PARP1delZn1-L713F

Plasmid

#173937

Purpose

Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn1-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 1-96; L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn3-L713F

Plasmid

#173934

Purpose

Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-G871L

Plasmid

#174798

Purpose

Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-G871L (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

G871L, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A870L

Plasmid

#174797

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A870L (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A870L, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-K893I

Plasmid

#174799

Purpose

Bacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-K893I (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

K893I, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_hTLN2-head-LBD

Plasmid

#164837

Purpose

Expression of human talin2 head mutant

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His8

Expression

Bacterial

Mutation

Lipid binding deficient mutations

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-GFP-tglG

Plasmid

#134679

Purpose

Expresses His6-GFP-TglG fusion in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglG

Tags

His6-GFP

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SznF E281A

Plasmid

#139591

Purpose

expresses SznF E281A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF E281A

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 281 to alanine

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SznF H225A

Plasmid

#139590

Purpose

expresses SznF H225A

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF H225A

Tags

hexahistidine

Expression

Bacterial

Mutation

changed histidine 225 to alanine

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SznF E215A

Plasmid

#139565

Purpose

expresses SznF E215A in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF E215A

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 215 to alanine

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43-NT-G46D

Plasmid

#134588

Purpose

Express human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)

Tags

His

Expression

Bacterial

Mutation

full-length (G154D)

Promoter

T7

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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