We narrowed to 5,458 results for: pcDNA3.1+
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Plasmid#158219PurposeBRET biosensor for detection of free Gβγ in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK3ct
ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-hACE2-LgBiT
Plasmid#215837PurposeExpresses hACE2 receptor with LgBiT tag in mammalian cellsDepositorInserthACE2-LgBiT (ACE2 )
ExpressionMammalianAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoA-91-NLuc
Plasmid#207151PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
ExpressionMammalianMutationNLuc inserted at position L(91)GAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαoB-91-NLuc
Plasmid#207152PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAO
ExpressionMammalianMutationNLuc inserted at position L(91)GAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22
Plasmid#159127Purposeexpresses FBXO22 in mammalian cellsDepositorAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg5_T
Plasmid#147552PurposeMammalian Expression of HsSmg5DepositorInsertHsSmg5 (SMG5 Human)
ExpressionMammalianMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dead-Laconic/pcDNA3.1(-)
Plasmid#118627PurposeTo express in mammalian cells a lactate FRET sensor negative control.DepositorInsertDead-Laconic
ExpressionMammalianMutationChange Histidine 151 to Aspartic Acid of Escheric…PromoterCMV PromoterAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi1-LgB91
Plasmid#134342PurposeNanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-pcDNA3.1-
Plasmid#52534Purposeenables eukaryotic expression of N-terminally RGS-6xHis–tagged fusion proteinsDepositorTypeEmpty backboneTagsRGS-6xHisExpressionMammalianPromoterCMVAvailable SinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-Tet1-CD
Plasmid#83341PurposeTo introduce MS2 coat protein fused Tet1-CDDepositorInsertMS2-Tet1-CD
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-Rac1(WT)
Plasmid#224278PurposeCell transfection and expression of Rac1DepositorInsertRac1 (RAC1 Human)
ExpressionMammalianAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLT3-V5/HIS
Plasmid#201985Purposeexpression of human FLT3 receptor tyrosine kinase in mammalian cellsDepositorAvailable SinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PKI-GFP
Plasmid#118482PurposeGFP-tagged Protein Kinase A inhibitor peptide.DepositorInsertPKI-GFP
TagsmEGFPExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-20nm-EBFP2
Plasmid#231185PurposeExpresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nmDepositorInsert20nm-EBFP2
ExpressionMammalianPromoterCMVAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7A-mCherry
Plasmid#62635Purposemammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
ExpressionMammalianMutationmCherry inserted into M3-M4 loopPromoterCMVAvailable SinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-3xFLAG
Plasmid#214984PurposeGateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tagDepositorTypeEmpty backboneTags3xFLAGExpressionMammalianAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi3-91-NLuc
Plasmid#207150PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi2-LgB91
Plasmid#134358PurposeNanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX
Plasmid#52421PurposeExpresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherryDepositorInsertFusion NLS-EGFP-P2A-Cherry-CAAX
ExpressionMammalianPromoterCMVAvailable SinceApril 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/ZMYM2-FGFR1
Plasmid#167486PurposeMammalian expression vector for the ZMYM2-FGFR1 fusion gene associated with myeloid and lymphoid neoplasmsDepositorAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only