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pLL3.7m-psMEK1tight

Plasmid

#89362

Purpose

A lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK1tight (MAP2K1 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-RUNX1-4D

Plasmid

#238535

Purpose

Used for overexpression of human RUNX1 in mammalian cells. Contains RUNX1 phosphomimetic mutations.

Depositor

Diane Krause

Article

Kwon et al Blood. 2024 Oct 24;144(17):1800-1812. do

Inserts

RUNX1 (RUNX1 Human)
Puromycin

Use

Lentiviral

Expression

Mammalian

Mutation

S249D, S266D, T273D, S276D

Promoter

MSCV and PGK

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psMEK1

Plasmid

#89361

Purpose

A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK1 (MAP2K1 Synthetic, Human)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLXSN IkB alpha M

Plasmid

#12330

Depositor

Inder Verma

Article

Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

Insert

IkB alpha M (Nfkbia Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Dominant-negative mutant. Many signal transductio…

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pSNV2 CHER NLS cop1at(335-675)

Plasmid

#44972

Depositor

Thanos Halazonetis

Article

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

mCherry-NLS-COP1 (COP1 Mustard Weed)

Tags

NLS and mCherry

Expression

Mammalian

Mutation

deleted amino acids 1-334

Promoter

pCMV IE

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only

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SpIMPDH

Plasmid

#206980

Purpose

Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

Depositor

Vern Schramm

Article

Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

Insert

SpIMPDH (guaB Streptococcus pyogenes)

Tags

His tag (with TEV cleavage site)

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MKK7a1-EE

Plasmid

#47578

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK7a1-EE (Map2k7 Mouse)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Residues 73-420 of RefSeq sequence. Ser271 to Gl…

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pAdEasy-EGFP-CLASP2 340-1362 9xS/A

Plasmid

#24523

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1362) 9xS/A (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant. M…

Available Since

July 22, 2010

Availability

Academic Institutions and Nonprofits only
huCofilin S3A SSR

Plasmid

#245939

Purpose

Expresses human cofilin with mutation S3A that prevents cofilin's inactivation by phosphorylation of S3

Depositor

James Bamburg

Article

Agnew et al J Biol Chem. 1995 Jul 21;270(29):17582-7

Insert

cofilin 1 (cfl1) (CFL1 Human)

Expression

Mammalian

Mutation

S3A;silent mutations (NTs 67 to 75) in which TCTT…

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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VAMP3(S48A)-mCherry

Plasmid

#193635

Purpose

Expresses S48A phosphodead VAMP3 fused to mCherry

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

changed serine 48 to alanine

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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VAMP3(S48E)-mCherry

Plasmid

#193637

Purpose

Expresses S48E phosphomimetic VAMP3 fused to mCherry

Depositor

Geert van den Bogaart

Article

Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

changed serine 48 to alanine

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN12(D199A,C231S)-Clover

Plasmid

#215528

Purpose

Expression vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Tags

Clover

Expression

Mammalian

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pINDUCER20-PTPN12(D199A,C231S)

Plasmid

#215518

Purpose

Dox-inducible lentiviral transfer vector with a phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Lentiviral

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

mCMV, Doxycycline-inducible

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-PTPN12(D199A,C231S)-Clover

Plasmid

#215522

Purpose

Gateway entry vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Gateway Cloning

Tags

Clover

Mutation

D199A, C231S (Phosphatase inactive)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-TO-Δ37-HA PPM1H

Plasmid

#208372

Purpose

Lentiviral expression of HA-tagged human Δ37-PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

delta37-HA PPM1H (PPM1H Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta37

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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CSII-PPM1H-mApple-mito

Plasmid

#207351

Purpose

Mammalian expression of PPm1H-mito

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPm1H-mito (PPM1H Human)

Expression

Mammalian

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-PPM1H S124A

Plasmid

#208368

Purpose

Mammalian expression of HA-tagged human PPM1H S124A

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-PPM1H S124A (PPM1H Human)

Expression

Mammalian

Mutation

S124A

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-PPM1H S211A

Plasmid

#208369

Purpose

Mammalian expression of HA-tagged human PPM1H S211A

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-PPM1H S211A (PPM1H Human)

Expression

Mammalian

Mutation

S211A

Available Since

Dec. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ10 PPM1H

Plasmid

#207335

Purpose

Mammalian expression of HA-Δ10 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ10

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ20 PPM1H

Plasmid

#207336

Purpose

Mammalian expression of HA-Δ20 PPM1H

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ20

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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