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pJBEI-6410

Plasmid

#47049

Purpose

BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

Depositor

Taek Soon Lee

Publication

Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

Insert

Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene

Use

Synthetic Biology; Bglbrick

Expression

Bacterial

Mutation

R364S mutation in MK

Promoter

PlacUV5

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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3xERRE-luciferase

Plasmid

#37851

Depositor

Rebecca Riggins

Publication

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

3 copies of ERRE upstream of luciferase

Use

Luciferase

Promoter

SV40

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDONR223-EPHA2

Plasmid

#23926

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EPHA2 (EPHA2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pBEN276

Plasmid

#69150

Purpose

Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

Depositor

Pierre Germon

Publication

Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

Insert

luxCDABE

Use

Luciferase

Promoter

frr

Available since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PB_tre_dCas9_VP160

Plasmid

#126031

Purpose

PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

Depositor

Mauro Calabrese

Publication

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

dCas9 fusion with VP160
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

EF1 alpha and TRE

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hsyn-GRAB_Ado1.0

Plasmid

#153285

Purpose

Express the adenosine sensor GRAB_Ado1.0 in neurons

Depositor

Yulong Li

Publication

Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

Has service

AAV1

Insert

Adenosine sensor GRAB_Ado1.0

Use

AAV

Promoter

hSyn

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-hSOX9-1

Plasmid

#40644

Depositor

Bob Weinberg

Publication

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-hSOX9-1 (SOX9 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)

Plasmid

#129405

Purpose

circular Pepper RNA

Depositor

Samie Jaffrey

Publication

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

circular F30-Pepper(TAR Variant-2)

Expression

Mammalian

Promoter

U6

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRetroSuper-shSrc

Plasmid

#26982

Depositor

Joan Massague

Publication

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

shRNA Src (SRC Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pAAV-hysn-flex-dsRed-shscramble

Plasmid

#71383

Purpose

Expresses scramble shRNA Cre-dependently by flex switch

Depositor

William Wisden

Publication

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

human synapsin promoter-flex switch-dsRed-shscramble

Use

AAV, Cre/Lox, and RNAi

Expression

Mammalian

Promoter

human synapsin

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pPSU250

Plasmid

#173837

Purpose

Expresses 250 kD Penn State ladder protein

Depositor

Song Tan

Publication

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACIL1bQRSSTRHSTPABMBPpepN

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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peCALWY-4

Plasmid

#22236

Depositor

Maarten Merkx

Publication

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-4 Zn(II) sensor (ATOX1 GFP's, Human)

Expression

Mammalian

Mutation

C418S mutation linker consists of 9 GGSGGS repea…

Available since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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[#GS15-LV] DsRed GFP TEVp Blast Switch - Recipient

Plasmid

#233506

Purpose

Lentiviral construct for the MitoTRACER genetic reporter to be expressed in the recipient cells

Depositor

Simon Grelet

Publication

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

DsRed loxp eGFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR4_G3BP2

Plasmid

#127105

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP2 (G3BP2 Human)

Use

Gateway Cloning

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-WT

Plasmid

#133753

Purpose

Wild type human TDP-43 in pLVX-Puro lentiviral vector

Depositor

Shawn Ferguson

Publication

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDONR223-AXL

Plasmid

#23945

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AXL (AXL Human)

Use

Gateway Cloning

Mutation

Q31P, Q764R

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMSCVpuro-eGFP-mcGAS

Plasmid

#108675

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

Depositor

Andrew Jackson, Martin Reijns

Publication

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (Cgas Mouse)

Use

Retroviral

Promoter

LTR

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCW57.1-Luciferase

Plasmid

#99283

Purpose

All-in-one lentivector with tet-inducible luciferase

Depositor

Stephen Tapscott

Publication

Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

Insert

Firefly luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pGEX5X.1 PINK1 WT

Plasmid

#13321

Depositor

Mark Cookson

Publication

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 kinase domain (PINK1 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 112-496

Available since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mEos2-C1

Plasmid

#54510

Purpose

Localization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Type

Empty backbone

Tags

mEos2

Expression

Mammalian

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article