19,660 results

1. pUCmini-iCAP-AAV.CAP-Mac

   Plasmid

   #200658

   Purpose

   Synthetic construct isolate AAV.CAP-Mac VP1 gene

   Depositor

   Viviana Gradinaru

   Article

   Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

   Insert

   Synthetic construct isolate AAV.CAP-Mac VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   7 amino acid insertion after Q588 of the AAV9 CAP…

   Promoter

   p41

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAX APLP1

   Plasmid

   #30141

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Like Protein 1 (APLP1 Human)
   

   Expression

   Mammalian

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pEGFP-N1 human cofilin S3E

   Plasmid

   #50861

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEBG

   Plasmid

   #22227

   Purpose

   GST fusion vector

   Depositor

   David Baltimore

   Article

   Tanaka et al Mol Cell Biol. 1995 Dec . 15(12):6829-37

   Type

   Empty backbone

   Tags

   GST

   Expression

   Mammalian

   Available Since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BRD4

   Plasmid

   #38942

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BRD4 (BRD4 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAVS1-TetOn-dCas9-KRAB

   Plasmid

   #115545

   Purpose

   Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

   Depositor

   Rene Maehr

   Article

   Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

   Type

   Empty backbone

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   CAG

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223_H2AFX_WT

   Plasmid

   #81937

   Purpose

   Gateway Donor vector containing H2AFX, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   H2AFX (H2AX Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTPGI_dCas9

   Plasmid

   #64279

   Purpose

   encodes yeast-optimized dCas9 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   Yeast-optimized dCas9
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   D10A & H840A relative to S. pyogenes Cas9

   Promoter

   pTPGI

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKDsgRNA-NT

   Plasmid

   #89960

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   empty gRNA
   

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid

    #72325

    Purpose

    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SnoopTag-mEGFP-SpyTag
    

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-mNox4

    Plasmid

    #69353

    Purpose

    expresses mouse Nox4 in mammalian cells

    Depositor

    Karl-Heinz Krause

    Article

    Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

    Insert

    mNox4 (Nox4 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

12. TSHR-Tango

    Plasmid

    #66518

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    TSHR (TSHR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-SpdCas9-VP64

    Plasmid

    #115794

    Purpose

    Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    VP64
    

    Use

    AAV

    Tags

    3XFLAG and dCas9-VP64

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLAG-SENP2

    Plasmid

    #18047

    Depositor

    Edward Yeh

    Article

    Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

    Insert

    SENP2 (SENP2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Halo-Rab6A

    Plasmid

    #190171

    Purpose

    Expresses HaloTag-Rab6A in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    HaloTag-Rab6A
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CCR4-Tango

    Plasmid

    #66241

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CCR4 (CCR4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mKikGR-N1

    Plasmid

    #54756

    Purpose

    Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    mKikGR

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pSLC-217

    Plasmid

    #73163

    Purpose

    Template plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-relE (relE E. coli (bacteria))
    

    Expression

    Bacterial

    Promoter

    PrhaB

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Nsp6-mCherry

    Plasmid

    #165133

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp6 (ORF1ab codon optimized SARS-COV-2)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lox-Stop-Lox TOPO

    Plasmid

    #11584

    Purpose

    Cre-dependent activation of gene of interest by removing Stop sequences; Puro selection.

    Depositor

    Tyler Jacks

    Article

    Jackson et al Genes Dev. 2001 Dec 15. 15(24):3243-8.

    Insert

    loxP-STOP-loxP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge