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mCit(S1h-S3h)3-gs

Plasmid

#228076

Purpose

Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, S1h, S3h

Expression

Mammalian

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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mCit(D1h-D3h)3-gs

Plasmid

#228088

Purpose

Mammalian expression of coiled coil segments (D1h-D3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, D1h, D3h

Expression

Mammalian

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2h)12-gs

Plasmid

#228087

Purpose

Mammalian expression of coiled coil segments (S2h)x12, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2h

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP(S2h)3(S4h)3-gs

Plasmid

#228077

Purpose

Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

BFP, S2h, S4h

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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mFzd4CRDL(42-203)-6xHis_pAcGP67a

Plasmid

#216379

Purpose

Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

6xHis and GP64 signal sequence

Expression

Insect

Promoter

Polyhedrin

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-Crym-BioID2-BioID2

Plasmid

#200070

Purpose

To study u-crystallin protein interactors

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

GfaABC1D-Crym-BioID2-BioID2

Use

AAV and Mouse Targeting

Tags

HA-tag

Promoter

GfaABC1D

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-Crym-eGFP

Plasmid

#200080

Purpose

To study u-crystallin protein interactors, control plasmid

Depositor

Baljit Khakh

Article

Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

Insert

GfaABC1D-Crym-eGFP

Use

AAV and Mouse Targeting

Tags

eGFP

Promoter

GfaABC1D

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK2 RPS28 UTR1 SCR

Plasmid

#203158

Purpose

RPS28 UTR luciferase assay plasmid (SCR UTR1, sites 1 and 2)

Depositor

Susan Baserga

Article

Bryant et al Nucleic Acids Res. 2024 Feb 28;52(4):198

Insert

RPS28 UTR (RPS28 Human)

Use

Luciferase

Mutation

scrambled the nucleotides in the two MIR-28 bindi…

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CSNK1G1_IDG-K

Plasmid

#214458

Purpose

Gateway destination clone of CSNK1G1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CSNK1G1 (CSNK1G1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEK21

Plasmid

#210586

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdTUBA1pro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdTUBA1pro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEK23

Plasmid

#210595

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdH4pro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdH4pro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEK24

Plasmid

#210597

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdACTINpro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdACTINpro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204797

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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FB323

Plasmid

#203570

Purpose

Module for the expression of geneticin resistance and Nanoluciferase, and the expression of firefly luciferase under the Ppaf promoter

Depositor

Jose Marcos

Article

Gandia et al Microb Biotechnol. 2022 Feb;15(2):630-64

Insert

PgpdA:Nluc:Ttub::PtrpC:nptII:Ttub::Ppaf:Luc:Ttub

Use

Synthetic Biology

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pOET3-C-TEV-Twin-Strep

Plasmid

#203324

Purpose

Generate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site (ENLYFQG) and Twin-strep-tag

Expression

Insect

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-DmeIF4G_578-650_Z

Plasmid

#148183

Purpose

Bacterial Expression of DmeIF4G_578-650

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4G_578-650 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmeIF4G_578-650-GB1_Z

Plasmid

#148154

Purpose

Bacterial Expression of DmeIF4G_578-650

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4G_578-650 (eIF4G1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-AAGAB

Plasmid

#198333

Purpose

Expresses AAGAB-GFP fusion in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AAGAB (AAGAB Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAcrVIB1-cloDF-amp

Plasmid

#184843

Purpose

Expression of anti-CRISPR protein AcrVIB1 in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

anti-CRISPR protein inhibiting Cas13b nucleases

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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nPPARg429-gs10-NLS-VPR

Plasmid

#188266

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nPPARg429 (PPARG Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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