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LII ins 2-3

Plasmid

#54379

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

insulator sequence, dummy 2-3

Use

Cloning vector

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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LII ins 4-5

Plasmid

#54380

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

insulator sequence, dummy 4-5

Use

Cloning vector

Available Since

July 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-neo-μ2-(HA)3

Plasmid

#198178

Purpose

Expression of HA-tagged AP-2 μ2 in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-2 μ2

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 80

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-MCS1M(8)Kana-mCMV-pA

Plasmid

#22059

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

mCMV-pA expression cassette

Expression

Mammalian

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only
pQCXIP-Rx3(A6)-mEGFP

Plasmid

#35530

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Rx3(A6)mEGFP

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-StrepTag II-PuroR

Plasmid

#80967

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

StrepTag II

Use

Gene tagging

Promoter

none

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pFH24

Plasmid

#131763

Purpose

Level0 SpCas9, wheat codon optimized (version 2)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

SpCas9, wheat codon optimized, version 2

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRVdG-EGFP-N-P-M-SynPhRFP-L

Plasmid

#52479

Purpose

Expresses EGFP and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

EGFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C T-Saphire

Plasmid

#54424

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

T-saphhire + 16 aa GS linker

Use

Cloning vector

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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LI D-E 6xHIS

Plasmid

#54447

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

polyhistidine (6x) tag

Use

Cloning vector

Available Since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI D-E T-Saph + linker c1

Plasmid

#54412

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + T-Sapphire

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pFH92

Plasmid

#125820

Purpose

Level0 nScCas9-ABE7.10, wheat/human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nScCas9-ABE7.10, wheat/human codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pICH72400

Plasmid

#50338

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, Atug7 (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFH23

Plasmid

#128180

Purpose

Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SpCas9 (wheat codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH53

Plasmid

#128182

Purpose

Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-NT

Plasmid

#17594

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-NT (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 49-498

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pJUMP41-2A(sfGFP)

Plasmid

#126985

Purpose

Level 2 vector. sfGFP reporter. Origin R6K (pir conditional).

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LI B-C Cerulean

Plasmid

#54425

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

CFP-Cerulean + 16 aa GS linker

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C YFP

Plasmid

#54423

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

YFP-Venus + 16 aa GS linker

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL11197

Plasmid

#191784

Purpose

SpCas9 expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas9 +13 introns-T-E9

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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