176,531 results

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DHFR-SpCas9-DHFR

Plasmid

#85447

Purpose

Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Publication

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-SpCas9-DHFR

Use

AAV

Tags

Destabilized domain (C-terminus version) of E.col…

Expression

Mammalian

Promoter

Cbh

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACYC-Skp-GroEL/ES

Plasmid

#83922

Purpose

Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

Depositor

Karl Griswold

Publication

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Gro EL/ES

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLuc

Plasmid

#83281

Purpose

recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

Depositor

Mark Kay

Publication

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

Firefly Luciferase

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pCRISPaint-TagGFP2-PuroR

Plasmid

#80970

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagGFP2

Use

Gene tagging

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pIκBα-miRFP703

Plasmid

#80005

Purpose

IκBα fused with near-infrared fluorescent protein miRFP703 (IκBα reporter)

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

IκBα (NFKBIA Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3_erGAP3

Plasmid

#78118

Purpose

Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

Depositor

Teresa Alonso, Javier García-Sancho

Publication

Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

Insert

erGAP3

Tags

KDEL and calreticulin signal peptide

Expression

Mammalian

Mutation

S175G, D180Y, I167V and L15Q in GFP moiety and D…

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP_TRPM8

Plasmid

#64879

Purpose

Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

Depositor

Sebastián Brauchi

Publication

Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

Insert

Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)

Tags

eGFP

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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p323-L10A-PATagRFP

Plasmid

#74172

Purpose

Fluorescent reporter for single ribosome tracking

Depositor

Robert Singer

Publication

Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

Insert

ribosomal protein L10A (Rpl10a Rat)

Use

Lentiviral

Tags

PATagRFP

Promoter

UbC

Available since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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NLS-mCherry-NES reporter (pDN160)

Plasmid

#72660

Purpose

NLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158

Depositor

Barbara Di Ventura, Roland Eils

Publication

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

NLS-mCherry-NES

Expression

Mammalian

Promoter

CMV

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pET28a SnoopTag-mEGFP-SpyTag

Plasmid

#72325

Purpose

Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

Depositor

Mark Howarth

Publication

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SnoopTag-mEGFP-SpyTag

Tags

N-terminal His6 tag

Expression

Bacterial

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-MSP1D1deltaH4-H6

Plasmid

#71717

Purpose

Membrane scaffold protein (MSP), helix 4 to 6 deletion

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pST44

Plasmid

#64007

Purpose

polycistronic cloning vector for pST44 plasmid suite

Depositor

Song Tan

Publication

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pT040

Plasmid

#67640

Purpose

URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

Depositor

John Wyrick

Publication

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Insert

single guide RNA expression cassette

Use

CRISPR

Expression

Yeast

Promoter

pSNR52

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5FRT-EF-Pdgfrbeta-EGFPN

Plasmid

#66790

Purpose

Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Publication

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRbeta (Pdgfrb Mouse)

Tags

C-term EGFP

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Str-KDEL_neomycin

Plasmid

#65306

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end

Tags

KDEL motif and Signal Sequence

Expression

Mammalian

Promoter

CMV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CRISPR-SP-Cas9 reporter

Plasmid

#62733

Purpose

Mammalian CRISPR-SP-Cas9 reporter

Depositor

Niels Geijsen

Publication

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

CRISPR-SP-Cas9 target seq + tdTomato (out of frame)

Use

CRISPR and Lentiviral

Tags

CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

Expression

Mammalian

Promoter

EF1-Alpha

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pQE-80L MBP-SspB Nano

Plasmid

#60409

Purpose

E. coli expression of SspB Nano

Depositor

Brian Kuhlman

Publication

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

SspB nano

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

Y11K and A15E

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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PGK-AAVS1ZFNL

Plasmid

#60916

Purpose

encodes zinc finger nuclease specific to AAVS1 locus (left)

Depositor

Paul Gadue

Publication

Sim et al Methods Mol Biol. 2015 Jan 29.

Insert

AAVS1-ZFNL

Promoter

PGK

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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gRNA-eGFP-Reporter

Plasmid

#60719

Purpose

Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

Depositor

Charles Gersbach

Publication

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

CRISPR

Expression

Mammalian

Promoter

U6 and mouse U6

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCS2-Gal4ff.zf1

Plasmid

#61392

Purpose

mRNA synthesis of Gal4ff codon modified for zebrafish

Depositor

Harold Burgess

Publication

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Gal4ff.zf1

Use

Zebrafish expression

Promoter

sp6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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