We narrowed to 7,792 results for: RAP

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L2127-FRB-GPA-114-20-CyOFP1

Plasmid

#222886

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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L2129-FRB-GPA-114-5-CyOFP1

Plasmid

#222888

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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L2128-FRB-GPA-114-10-CyOFP1

Plasmid

#222887

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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L2131-FKBP-GPA-114-10-CyOFP1

Plasmid

#222890

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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L2130-FKBP-GPA-114-20-CyOFP1

Plasmid

#222889

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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APCDD1-P2A-tdTomato

Plasmid

#210801

Purpose

Fluorescent reporter for APCDD1 expression

Depositor

Yuejun Chen

Article

You et al Cell Stem Cell. 2023 Apr 6;30(4):473-487

Insert

APCDD1 (APCDD1 Human)

Tags

tdtomato

Expression

Mammalian

Promoter

endougeneous APCDD1 promoter

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-FKBP-LBR

Plasmid

#222427

Purpose

To express LBR with N-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR (LBR Human)

Tags

GFP-FKBP

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-BE

Plasmid

#193648

Purpose

Expression of base editor dCas12a-BE in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-rAPOBEC1, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP12-CB2mutx2-mTagBFP2-HAx2

Plasmid

#207147

Purpose

Tandem mutant calcium-buffer with rapalog dimerizer

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

Calbindin-2 (CALB2 Human)

Tags

FKBP12, HA, and mTagBFP2

Expression

Mammalian

Mutation

E40Q, E87Q, E131Q, E175Q, E219Q

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL1RN gRNA_1-MS2-Puro

Plasmid

#192677

Purpose

Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNA #1 (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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DHN_VrDHN1a_mCh-2xFKBP (pBS1117)

Plasmid

#185312

Purpose

For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_VrDHN1a

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_DHN_VrDHN1a (or YSK2) (pBS0807)

Plasmid

#185266

Purpose

For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a (or YSK2). This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_VrDHN1a (or YSK2)

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-RanGAP*

Plasmid

#175238

Purpose

Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Mutation

Amino acid 562 F to A

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-HF2-BE3

Plasmid

#120397

Purpose

Expresses HF2-BE3 in mammalian cells

Depositor

Junjiu Huang

Article

Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

Insert

HF2-BE3

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-FKBP-DCRD

Plasmid

#87453

Purpose

rapamycin-induced membrane-targeting of DCRD

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

DCRD (distal C-terminal regulatory domain) (CACNA1F Human)

Tags

6xHis-T7, FKBP, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-FKBP-PCRD

Plasmid

#87452

Purpose

rapamycin-induced membrane-targeting of PCRD

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

PCRD (proximal C-terminal regulatory domain) (CACNA1F Human)

Tags

FKBP and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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SRAcode_in_pUC57_v3(419-999)

Plasmid

#49824

Purpose

for transcribing SRA nucl 419-999 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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SRAcode_in_pUC57_v3(767-999)

Plasmid

#49825

Purpose

for transcribing SRA nucl 767-999 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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SRAcode_in_pUC57_v3(419-771)

Plasmid

#49823

Purpose

for transcribing SRA nucl 419-771 of NM_001035235 with T7

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator RNA coding (SRA1 Human)

Expression

Bacterial

Mutation

KpnI site at 5' start of RNA coding seq

Promoter

T7

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pTRE-BI-TRIM21

Plasmid

#207838

Purpose

Inducible TRIM21 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Article

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

TRIM21 (TRIM21 Human)
mCherry
hIgG1-FC
VHH-GFP4

Tags

weak NLS

Expression

Mammalian

Mutation

All K mutated to R

Promoter

TRE3G BI promoter

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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