We narrowed to 9,347 results for: tre promoter
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Plasmid#82697PurposeFor targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.DepositorTypeEmpty backboneUseRNAiPromoterhPGK, U6Available SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only
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AAV-hSyn-SpdCas9-W3SL
Plasmid#210708PurposeThis Plasmid express hSyn promoter driven SpdCas9DepositorInsertS. Pyogenes dCas9
UseAAV and CRISPRTagsFLAGExpressionMammalianMutationD10A/H840APromoterhSynAvailable SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
hPGK_V5-APEX2-GFP
Plasmid#105285Purposedrives expression of V5-NES-APEX2-GFP from human PGK promotorDepositorInsertV5-APEX2-NES
TagsGFPExpressionMammalianPromoterhuman PGK promoterAvailable SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-tdTomato
Plasmid#199250PurposeFluorescent labelling of bacterial cells with tdTomatoDepositorInsertCodon-optimized tdTomato gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-His6MBP-A3A-Gly6His6
Plasmid#187822PurposeExpression human APOBEC3A in E.coli.DepositorInsertAPOBEC3A (APOBEC3A Human)
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationnonePromoterT7 promoter (on the backbone)Available SinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mCherry
Plasmid#199247PurposeFluorescent labelling of bacterial cells with mCherryDepositorInsertCodon-optimized mCherry gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAaePUb::Cas9-2A-Neo
Plasmid#176680PurposeExpression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)DepositorInsertspCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
UseCrisprExpressionInsectMutationDrosophila codon optimizedPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLex_Cas9
Plasmid#117987PurposepLex_Cas9 is a single insert lentiviral vector expressing Cas9 driven by CMV promoter.DepositorInsertCas9-P2A-NLS-BASTR
UseLentiviralTagsP2A linker, nuclear localization signal and blast…Available SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-Pc-mKate2
Plasmid#199249PurposeFluorescent labelling of bacterial cells with mKate2DepositorInsertCodon-optimized mKate2 gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPSt_35Spr-GFP-t35S
Plasmid#203592PurposeFully assembled Plant STARR-seq plasmid with the 35S terminator.DepositorInsertsCaMV 35S promoter + Zm00001d041672 5'UTR
eGFP
CaMV 35S terminator
ExpressionPlantAvailable SinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1
Plasmid#192470PurposeTransient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS8
TagsAcGFP1ExpressionInsectPromoterAc5 promoterAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1
Plasmid#192469PurposeTransient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS3
TagsAcGFP1ExpressionInsectPromoterAc5 promoterAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
SECURE miniABEmax-K20A/R21A (pJUL1774)
Plasmid#131312PurposeCMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).DepositorInsertbpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
ExpressionMammalianPromoterCMVAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RHO-IntC-dCas9C-VPR-polyA
Plasmid#166692PurposeExpress C-terminal part of split dCas9-VPRDepositorInsertIntC-dCas9C-VPR
UseAAV and CRISPRExpressionMammalianPromotershort human rhodopsin promoterAvailable SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-BcPC-PLC
Plasmid#224255PurposeExpress N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coliDepositorTagsGST and HRV-3C (LEVLFQGP) siteExpressionBacterialMutationThe gene of Bacillus cereus ATCC 14579 was used f…Promoterlac promoterAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEVA228-pro4IUPi
Plasmid#122018PurposeExpresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.DepositorInsertsExpressionBacterialMutationCodon optimized for E. coliPromoterpro4Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL11060
Plasmid#68264PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)DepositorInsertPromoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyExpressionPlantAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a-Nlux
Plasmid#176239PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferasePromoterRibosomal subunitAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC-BAR-YR
Plasmid#61766PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).DepositorInsertsBar gene
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…PromotertrpC promoterAvailable SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hlbCas12a-Nlux
Plasmid#176240PurposepNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized lbCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferasePromoterRibosomal subunitAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only