We narrowed to 13,276 results for: cDNA

1. pGP-CMV-jGCaMP8f L27A

   Plasmid

   #204140

   Purpose

   Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f L27A
   

   Use

   Tags

   N-terminal His tag

   Expression

   Mammalian

   Mutation

   Leucine 27 changed to Alanine

   Promoter

   CMV

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. ALIX(P801G)-mNeonGreen

   Plasmid

   #191192

   Purpose

   Full-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen.

   Depositor

   Jin Zhang

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   ALIX (P801G) (PDCD6IP Human, Synthetic)
   

   Use

   Tags

   6xHIS and mNeonGreen

   Expression

   Mammalian

   Mutation

   Pro 801 mutated to Gly

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. sfGFP-SasG(7G)-mCherry

   Plasmid

   #206949

   Purpose

   Mammalian expression of heterobifunctional SasG linkers (7G)

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   sfGFP-SaSasG (7G)-mCherry
   

   Use

   Tags

   mCherry and sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sfGFP-SasG(6G)-mCherry

   Plasmid

   #206948

   Purpose

   Mammalian expression of heterobifunctional SasG linkers (6G)

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   sfGFP-SaSasG (6G)-mCherry
   

   Use

   Tags

   mCherry and sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. sfGFP-SasG(5G)-mCherry

   Plasmid

   #206947

   Purpose

   Mammalian expression of heterobifunctional SasG linkers (5G)

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   sfGFP-SaSasG (5G)-mCherry
   

   Use

   Tags

   mCherry and sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. sfGFP-SasG(4G)-mCherry

   Plasmid

   #206946

   Purpose

   Mammalian expression of heterobifunctional SasG linkers (4G)

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   sfGFP-SaSasG (4G)-mCherry
   

   Use

   Tags

   mCherry and sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sfGFP-SasG(2G)-mCherry

   Plasmid

   #206944

   Purpose

   Mammalian expression of heterobifunctional SasG linkers (2G)

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   sfGFP-SaSasG (2G)-mCherry
   

   Use

   Tags

   mCherry and sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-EGFP-DmMextli_577-620_AB

   Plasmid

   #148337

   Purpose

   Insect Expression of DmMextli_577-520

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

   Insert

   DmMextli_577-520 (mxt Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-EGFP-DmMextli_577-640_AB

   Plasmid

   #148338

   Purpose

   Insect Expression of DmMextli_577-640

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

   Insert

   DmMextli_577-640 (mxt Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pnEA-NpM-DmMextli_577-620_AB

    Plasmid

    #148355

    Purpose

    Bacterial Expression of DmMextli_577-620

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

    Insert

    DmMextli_577-620 (mxt Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pnEA-NpM-DmMextli_577-640_AB

    Plasmid

    #148360

    Purpose

    Bacterial Expression of DmMextli_577-640

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

    Insert

    DmMextli_577-640 (mxt Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETM-41-DmPABPC1_J

    Plasmid

    #146643

    Purpose

    Bacterial Expression of DmPABPC1

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    DmPABPC1 (pAbp Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    4 silent mutations compared to the sequence geven…

    Promoter

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

    Plasmid

    #169550

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

    Plasmid

    #169597

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9 - VP64 fusion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTL464

    Plasmid

    #69881

    Purpose

    a dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORF

    Depositor

    Tobias Langenhan, Robert Kittel

    Article

    Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

    Insert

    CIRL (Cirl Fly)
    

    Use

    Phic31-integration vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVH19-Tier1-PhCMV-p300core

    Plasmid

    #169572

    Purpose

    Tier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P300core (EP300 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTS2304-Tier1-Tactb-PPGK-NLuc_TB

    Plasmid

    #169549

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTS1046-Tier1-PhCMV-4xCox8-mRuby2

    Plasmid

    #169537

    Purpose

    Tier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTS2380-Tier1-PhCMV-TM

    Plasmid

    #169485

    Purpose

    Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) (PhCMV-TM-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Junctophilin 2 transmembrane domain (JPH2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCh-2xFKBP (no insert) (pBS1139)

    Plasmid

    #185322

    Purpose

    The empty vector used for the FKBP experiments in our paper

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

21. BirA-Myc_N_FOXI1

    Plasmid

    #167702

    Purpose

    Expresses FOXI1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    FOXI1 (FOXI1 Human)
    

    Use

    Tags

    Myc-BirA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGlomyc-Ovol1

    Plasmid

    #172871

    Purpose

    Mammalian expression vector with myc-tagged mouse Ovol1 coding sequence

    Depositor

    Renee van Amerongen

    Article

    Mourao et al bioRxiv 2021.06.22.449461

    Insert

    Ovol1 (Ovol1 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid

    #169743

    Purpose

    CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    mScarlet-I
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Equalizer-M plasmid with onboard mCherry cassette

    Plasmid

    #169734

    Purpose

    Equalizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CMV BARK R106A p2A mCherry

    Plasmid

    #117688

    Purpose

    CMV-iBARK(R106A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs R106A peptide
    

    Use

    Tags

    HA / FLAG

    Expression

    Mammalian

    Mutation

    R106A

    Promoter

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGAL4-DBD-OLIG2wt-IDR

    Plasmid

    #145250

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and OLIG2wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    OLIG2wt-IDR (OLIG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGAL4-DBD-MNX1wt-IDR

    Plasmid

    #145248

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and MNX1wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    MNX1wt-IDR (MNX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PTPRA_C433S-SHC

    Plasmid

    #139612

    Purpose

    StrepIII-HA-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    PTPRA_C433S (PTPRA Human)
    

    Use

    Tags

    StrepIII-HA

    Expression

    Mammalian

    Mutation

    C433S

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EGFP-Mm_Plk5(D168A)

    Plasmid

    #136318

    Purpose

    Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (Plk5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D168A

    Promoter

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EGFP-Hu_Plk5(W207F)

    Plasmid

    #136320

    Purpose

    Mammalian expression of human Plk5 W207F with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (PLK5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W207F

    Promoter

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge