We narrowed to 9,405 results for: pho;

1. pGem-sHyper:sAPX

   Plasmid

   #84738

   Purpose

   Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Inserts

   sAPX (SAPX Mustard Weed)
   sHyper (HyPer2 targeted to nucleus)
   

   Tags

   nuclear localisation signals from the coding sequ…

   Expression

   Plant

   Promoter

   pFMV/p35S

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Epac-S-H159

   Plasmid

   #170347

   Purpose

   mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-PGK-Chst3

   Plasmid

   #193024

   Purpose

   AAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoter

   Depositor

   Bart Nieuwenhuis

   Article

   Yang et al Mol Psychiatry. 2021 Jul 16. pii: 10.103

   Insert

   Chst3 (Chst3 Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and myc-tag

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42

   Plasmid

   #165436

   Purpose

   Expresses ShadowY-CBD(H83L/H86L) and Clover(T153M/F223R)-Cdc42 in mammalian cells as a negative control for FLIM imaging.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   ShadowY-CBD(H83L/H86L)-P2A-Clover(T153M/F223R)-Cdc42
   

   Tags

   ShadowY, Clover(T153M/F223R)

   Expression

   Mammalian

   Mutation

   H83L/H86L: CDB(Cdc42 Binding Domain, Pak3, residu…

   Promoter

   CMV

   Available Since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

   Plasmid

   #118412

   Purpose

   Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.

   Depositor

   Nicole Paulk

   Article

   Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1

   Insert

   Firefly luciferase
   

   Use

   AAV, Luciferase, and Synthetic Biology

   Expression

   Insect and Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGem-nHyPer:sAPX

   Plasmid

   #84735

   Purpose

   Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Inserts

   sAPX (SAPX Mustard Weed)
   nHyper (HyPer2 targeted to nucleus)
   

   Tags

   nuclear localisation signals from the coding sequ…

   Expression

   Plant

   Promoter

   FMV/35S

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV hSyn mCh-IRES-SspB(micro)-BoNT/B(147-441, Y365A)

   Plasmid

   #122986

   Purpose

   AAV plasmid with human synapsin promoter driving mCh and SspB(micro)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT

   Depositor

   Matthew Kennedy

   Article

   Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

   Insert

   mCh-IRES-SspB(micro)-BoNT/B(147-441)
   

   Use

   AAV

   Tags

   mCh

   Expression

   Mammalian

   Mutation

   SspB contains R73Q "micro" mutation, Bo…

   Promoter

   human synapsin

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTriEx-mVenus-PA-Rac1-C450A

   Plasmid

   #22021

   Purpose

   Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
   

   Tags

   6X His and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV domain contains the light-insensitive C450A m…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

   Plasmid

   #131143

   Purpose

   Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   mNeonGreen::3XFLAG::Crk (Crk Fly)
   

   Use

   Cre/Lox; Attb recombination site

   Tags

   3XFLAG and mNeonGreen

   Expression

   Insect

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-W

    Plasmid

    #208548

    Purpose

    Lentiviral vector expressing gRNA targeting human GATA3

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GATA3(13) (GATA3 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNA5(GATA3(12))-PGKpuro2ABFP-W

    Plasmid

    #208547

    Purpose

    Lentiviral vector expressing gRNA targeting human GATA3

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    GATA3(12) (GATA3 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-syn-FLEX-axon-jYCaMP1s

    Plasmid

    #135421

    Purpose

    Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Has Service

    AAV1

    Insert

    jYCaMP1s
    

    Use

    AAV

    Tags

    GAP43 axon targeting sequence

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. S10A mutant of H3S10ph biosensor

    Plasmid

    #120810

    Purpose

    FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
    

    Expression

    Mammalian

    Mutation

    Serine 10 is mutated to Alanine, and Threonine 3,…

    Promoter

    CMV

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-hPC1_4106-eGFP-3HA

    Plasmid

    #108399

    Purpose

    Express cytoplasmic tail human PC1 from amino acid 4106 to 4302

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-CD16.7-hPC1-eGFP-3HA

    Plasmid

    #108401

    Purpose

    Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

    Plasmid

    #102594

    Purpose

    Lentivector to express STING V155M delta 342 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M delta 342

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3 2198

    Plasmid

    #49347

    Purpose

    This is actually plasmid pGL3 2185 from the associated publication, which contains a shorter Bmp2 promoter sequence. also contains luciferase reporter.

    Depositor

    Stephen Harris, Melissa Rogers

    Article

    Feng et al Biochim Biophys Acta. 1997 Jan 3;1350(1)

    Insert

    Bmp2 promoter (shorter sequence) (BMP2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Promoter

    Bmp2 promoter

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTH734-2µ-RLuc/stopCFLuc

    Plasmid

    #40609

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase (nonsense mutant)
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCC336

    Plasmid

    #110167

    Purpose

    miniMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

    Depositor

    Iva Greenwald

    Article

    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

    Insert

    ERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B
    

    Use

    Minimos transposon

    Tags

    mCherry and mClover

    Expression

    Worm

    Mutation

    Phospho-acceptor sites mutated: S43E, T55E, T62E.…

    Promoter

    lin-31 promoter/enhancer

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCC335

    Plasmid

    #110166

    Purpose

    miniMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)

    Depositor

    Iva Greenwald

    Article

    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

    Insert

    ERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
    

    Use

    Minimos transposon

    Tags

    mCherry and mClover

    Expression

    Worm

    Mutation

    Phospho-acceptor sites mutated: S43A, T55A, T62A.…

    Promoter

    lin-31 promoter/enhancer

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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