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6xHis-SNAP-PI(3,5)P2 Probe

Plasmid

#211512

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SnxA-2xPX

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3,4,5)P3 Probe

Plasmid

#211514

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

BTK1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MbPylRS(DiZPK)

Plasmid

#91706

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

Depositor

P. Chen

Article

Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

Inserts

pylS
pylT

Expression

Mammalian

Mutation

L274A, C313S

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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plenti hSyn PGK1

Plasmid

#220919

Purpose

Neuronal specific expression of PGK1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1 (PGK1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hSyn

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mNeonGreen

Plasmid

#99134

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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p5E-0.349cldn15la

Plasmid

#125026

Purpose

Five prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.

Depositor

John Rawls

Article

Murdoch et al PLoS Pathog. 2019 Mar 7;15(3):e1007381.

Insert

Claudin 15-like a (cldn15la Zebrafish)

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-ExRai-CKAR2

Plasmid

#236098

Purpose

Plasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

Lyn-ExRai-CKAR2

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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WT (AF20)

Plasmid

#21359

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pREDTAI

Plasmid

#51627

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

trc

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-CAG-FLEX-BFP2-(mCherry)’

Plasmid

#115234

Purpose

Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

mTagBFP2 and mCherry

Expression

Mammalian

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-MBP-2xNLS-tdTomato

Plasmid

#104054

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-tdTomato

Use

AAV

Tags

NLS

Promoter

MBP

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

Plasmid

#153004

Purpose

Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (N) (G78)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

Plasmid

#153005

Purpose

Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (C) (G79)

Use

Lentiviral

Tags

FRB, HA, and HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-opto-hRIP3 dRHIM

Plasmid

#208788

Purpose

Inducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2

Depositor

Petr Broz

Article

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

RIP3 (RIPK3 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Mutation

RHIM domain is removed

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAL119-TK

Plasmid

#21911

Depositor

Maria Castro

Article

Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

Insert

HSV Type 1 thymidine kinase

Use

Adenoviral

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mTurquoise2

Plasmid

#99122

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mTurquoise2 from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti EF1a GFP-mCherry-mPlin1

Plasmid

#189005

Purpose

Fluorescent reportor for lipophagy

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

GFP, mChery, Plin1 (Plin1 Mouse)

Use

Lentiviral

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-2xNLS-mTurquoise2

Plasmid

#104053

Purpose

An AAV genome encoding expression of the nuclear localized fluorescent protein mTurquoise2 from the GFAP promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-mTurquoise2

Use

AAV

Tags

NLS

Promoter

GFAP

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB502

Plasmid

#74844

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Pak1 K299R

Plasmid

#12210

Depositor

Jonathan Chernoff

Article

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Catalytically inactive Pak1: K299R

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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