We narrowed to 17,159 results for: REV

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pD649-HAsp-LILRB4-COMP5AP-AviTag-9xHis

Plasmid

#157256

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LILRB4 (LILRB4 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFSF12-COMP5AP-AviTag-9xHis

Plasmid

#157187

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFSF12 (TNFSF12 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFSF14-COMP5AP-AviTag-9xHis

Plasmid

#157149

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFSF14 (TNFSF14 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_I770A_6xH

Plasmid

#185711

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed I770 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53

Plasmid

#187578

Purpose

Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

WRAP53 (WRAP53 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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HMGN1_pLENTI-CAG-IRES-GFP

Plasmid

#176995

Purpose

Mammalian lentiviral expression vector encoding HMGN1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

HMGN1 (HMGN1 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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Sufu_pLENTI-CAG-IRES-GFP

Plasmid

#176993

Purpose

Mammalian lentiviral expression vector encoding Sufu

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

Sufu (Sufu Mouse)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAT9751-BEAR-mCherry-preedited

Plasmid

#162993

Purpose

BEAR control plasmid with split mCherry and intact 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mCherry split with an intron between amino acids 119-120

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046277

Plasmid

#145428

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBa.GFP-KIF17 tail 400-1038

Plasmid

#134617

Purpose

Mammalian expression of mouse KIF1A 400-1038

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF17 (Kif17 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 399 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-161K enhancer-NR2F2-MUT-minP:fLuc-TK:rLuc

Plasmid

#138368

Purpose

Lentiviral plasmid contains 161k modified human enhancer with with NR2F2 binding sites mutated driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

161K human enhancer, with NR2F2 binding sites mutated, driving firefly enhancer and TK driving renilla luciferase

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCAD54_CAD29_H104A_pvp008

Plasmid

#124876

Purpose

Expresses hCAD His103Ala mutant in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

mutated His103 to Ala, deleted amino acids 1-3 an…

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL1 G116A

Plasmid

#123084

Purpose

Gateway entry clone encoding human GABARAPL1 G116A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Gateway Cloning

Mutation

Glycine 116 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAP G116

Plasmid

#123082

Purpose

Gateway entry clone encoding human GABARAP G116

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Gateway Cloning

Mutation

Deleted amino acid 117. Stop codon after G116

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 LC3A G120

Plasmid

#123071

Purpose

Gateway entry clone encoding human MAP1LC3A G120

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway Cloning

Mutation

Deleted amino acid 121. Stop codon after G120.

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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PB09-TRE-ZNF787_G379A

Plasmid

#117320

Purpose

Overexpression of ZNF787

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

ZNF787 (ZNF787 Human)

Use

Transposon-mediated integration

Expression

Mammalian

Mutation

G379A

Promoter

Tet-inducible promoter

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 T134R-pcDNA3.1

Plasmid

#118211

Purpose

Expresses FLAG-OTUB1 T134R mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Tags

3x FLAG

Expression

Mammalian

Mutation

changed threonine 134 to arginine

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-hSyntenin2b

Plasmid

#89437

Purpose

mammalian expression of EGFP tagged human Syntenin2b

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

*see below

Promoter

CMV

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-zic2a P

Plasmid

#89385

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant

Plasmid

#72922

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA Binding Protein 6 (GATA6 Human)

Use

Gateway Cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Promoter

TRE tight

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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