We narrowed to 19,063 results for: REV;

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pcDNA3-SypHluorin 2x (S2x)

Plasmid

#37004

Depositor

Stephen Heinemann, Yongling Zhu

Article

Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Insert

SypHluorin 2x (Syp Rat)

Tags

HA

Expression

Mammalian

Mutation

Two copies of pHluorin inserted at site 184-185

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pBS-PtH2B

Plasmid

#133390

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pME-NR2F2

Plasmid

#138359

Purpose

Gateway compatible middle entry clone contains human full length NFR2F2 gene with stop codon

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

Human NR2F2

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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LT2_7_OxPro

Plasmid

#177709

Purpose

Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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Halo-ATG9A HRD

Plasmid

#203222

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG9 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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560HTR

Plasmid

#24444

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis

Expression

Bacterial

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pXCas9H840A

Plasmid

#60900

Purpose

Dual Expression Vector for Cas9 H840A nickase and gRNA

Depositor

Bruce Conklin

Article

Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CBh and U6

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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AAVS1 LAMP1-mNeonGreen HRD

Plasmid

#207549

Purpose

Homologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible LAMP1-mNeonGreen

Tags

mNeonGreen

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD063

Plasmid

#212916

Purpose

Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT

Plasmid

#90244

Purpose

Expresses YFP-HA-DAT in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2006 Aug 2;26(31):8195-205.

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Expression

Mammalian

Mutation

residues 193–203 of human DAT were replaced in YF…

Promoter

CMV

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK-3xFLAG

Plasmid

#163452

Purpose

lentiviral overexpression vector for human NADK

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

NADK (NADK Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available Since

March 4, 2021

Availability

Academic Institutions and Nonprofits only

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pShuttle-HA-FLAG-TIN2

Plasmid

#35025

Depositor

Sheila Stewart

Article

Nittis et al Mol Cell Proteomics. 2010 Jun;9(6):1144-

Insert

TIN2 (TINF2 Human)

Use

Adenoviral

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag

Plasmid

#231601

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag under CMV promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag

Expression

Mammalian

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pFvL106

Plasmid

#64751

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, URA selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-JNKAR1

Plasmid

#61625

Purpose

FRET-based biosensor for monitoring JNK activity

Depositor

Jin Zhang

Article

Fosbrink et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

JNKAR1

Tags

Citrine and ECFP

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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Halo-ATG16L1 HRD

Plasmid

#207537

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAT15489_dABE8e

Plasmid

#174127

Purpose

plasmid expressing ABE8e with dCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

dABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-M2-TLR2

Plasmid

#12291

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I and H202Y; signal peptide (aa1-23) replaced …

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
CD4-MKII-f-hLOV1-TEVcs(ENLYFQ/M)- Gal4

Plasmid

#163027

Purpose

FLiCRE TF with fast kinetics for HEK293T expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

CD4-MKII-f-hLOV1-TEVcs-Gal4

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCPP6534

Plasmid

#128735

Purpose

Gateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tag

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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