19,660 results

1. CMV-GEM-GECO1

   Plasmid

   #32442

   Purpose

   Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEM-GECO1
   

   Expression

   Mammalian

   Mutation

   GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

   Promoter

   CMV

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCrl

   Plasmid

   #122443

   Purpose

   Expresses entire mouse reelin ORF in mammalian cells

   Depositor

   Tom Curran

   Article

   D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

   Insert

   reelin (Reln Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3X FLAG-FUS-WT

   Plasmid

   #44985

   Depositor

   Robin Reed

   Article

   Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

   Insert

   FUS (Full length) (FUS Human)
   

   Tags

   3X FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLENTI_hACE2_HygR

   Plasmid

   #155296

   Purpose

   Lentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistance

   Depositor

   Raffaele De Francesco

   Article

   Notarbartolo et al Sci Immunol. 2021 Aug 10;6(62). pii: 6/6

   Insert

   hACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   C9

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pEF6a-CD28-PafA

   Plasmid

   #113400

   Purpose

   Expresses CD28-PafA in mammalian cell

   Depositor

   Min Zhuang

   Article

   Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

   Insert

   CD28-PafA
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   EF-1α

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSXneo 270(T2AG3)

   Plasmid

   #12403

   Depositor

   Titia de Lange

   Article

   Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

   Insert

   270(T2AG3)
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-HA AKT1

   Plasmid

   #78778

   Purpose

   To overexpress AKT1 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

   Insert

   AKT1 (AKT1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS3Clux

   Plasmid

   #55172

   Purpose

   lux-reporter vector, integration at sacA, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Promoter

   none

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNTI194

   Plasmid

   #84266

   Purpose

   Expresses Avi-tagged RPL1 and humanized birA

   Depositor

   Nicholas Ingolia

   Article

   Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

   Inserts

   RPL1 (RPL10A Human, (*see below))
   humanized birA
   

   Tags

   Avi tag, Flag, Gly/Ser linker, and TEV site

   Expression

   Mammalian

   Mutation

   R33A, A34E (*see below)

   Promoter

   CMV and P2A peptide

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.cTNT.iCre

    Plasmid

    #69916

    Purpose

    Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.

    Depositor

    William Pu

    Article

    Lin et al Circ Res. 2015 Jan 2;116(1):35-45. doi:

    Has Service

    AAV9

    Insert

    improved cre (iCre) recombinase and tdTomato
    

    Use

    AAV

    Tags

    The iCre coding frame and tdTomato coding frame i…

    Promoter

    Chicken cardiac troponin T

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-MS2V5-PABPC1

    Plasmid

    #65807

    Purpose

    expression of PABPC1-MS2 fusion. MS2 tethers PABPC1 to mRNAs containing MS2 stem loops.

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    PAB1 (PABPC1 Human)
    

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. 35S-sfGFP-nosT

    Plasmid

    #80129

    Purpose

    Transient expression vector for sfGFP in plants

    Depositor

    Yutaka Kodama

    Article

    Fujii et al Plant Biotech. 2015; 32(1): 81-87.

    Type

    Empty backbone

    Tags

    sfGFP

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMitoLOC

    Plasmid

    #58980

    Purpose

    Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

    Inserts

    mCherry
    GFP
    

    Tags

    preCOX4 and preSU9

    Expression

    Yeast

    Promoter

    ADH1 and MET17

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRPML1-YFP

    Plasmid

    #18826

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML1 (MCOLN1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKS1

    Plasmid

    #84079

    Purpose

    Expresses the non-toxic fragment C of tetanus toxin

    Depositor

    Neil Fairweather

    Article

    Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

    Insert

    Tetanus neurotoxin fragment C
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a-TyrRS-His

    Plasmid

    #124121

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Tyrosyl-tRNA ligase (tyrS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJAM1.19_PB-CIT-PGK-Blast

    Plasmid

    #205564

    Purpose

    Express human CIT

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    CIT (CIT Human)
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. HC-M

    Plasmid

    #173481

    Purpose

    An rrnB P1-based GFP ATP reporter in E. coli

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

    Insert

    GFP-mut2
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Promoter

    rrnB P1

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tau (wt) -VC

    Plasmid

    #87369

    Purpose

    expresses hTau fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

    Insert

    MAPT (MAPT Human)
    

    Tags

    c-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. eMagA-EGFP-Mito

    Plasmid

    #162244

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagA-EGFP-OMP25
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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