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pGEX-4T-2 RanGAP

Plasmid

#13379

Depositor

Mary Dasso

Publication

Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

Insert

RanGAP (RANGAP1 Human)

Tags

GST

Expression

Bacterial

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pYES2-Ub-R-GFP

Plasmid

#11953

Depositor

Nico Dantuma

Publication

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-R-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET23b-Casp3 C163A-His

Plasmid

#11822

Depositor

Guy Salvesen

Publication

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C163A

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcFLAG-CSK-WT

Plasmid

#74502

Purpose

Overexpression of CSK in mammalian cells

Depositor

Lars Rönnstrand

Publication

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Tags

FLAG

Expression

Mammalian

Mutation

K222R

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Tipin

Plasmid

#22889

Depositor

Aziz Sancar

Publication

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pRR-ERbeta-5Z

Plasmid

#23062

Depositor

Charles Miller

Publication

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERbeta (ESR2 Human)

Expression

Yeast

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only
pCEP4-myc-HLA-B7

Plasmid

#135509

Purpose

Mammalian expression of myc-tagged HLA-B*07:02

Depositor

Erik Procko

Publication

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*07:02 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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CDK1as_T2A_Zeo

Plasmid

#118596

Purpose

One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.

Depositor

William Earnshaw

Publication

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

CDK1

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBabe-lincRNA-RoR

Plasmid

#45763

Depositor

George Daley

Publication

Loewer et al Nat Genet. 2010 Dec;42(12):1113-7. Epub

Insert

lincRNA-RoR cDNA (LINC-ROR Human)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-Ha-Brca1 S1423A

Plasmid

#32533

Depositor

Michael Kastan

Publication

Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BRCA1 (BRCA1 Human)

Tags

HA

Expression

Mammalian

Mutation

S1423A

Promoter

CMV/rabbit B-globin

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pFU-AgaIVA-VG

Plasmid

#24145

Depositor

Ines Ibanez-Tallon

Publication

Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

Insert

Venus-AgatoxinIVA-FLAG-GPI anchor

Use

Lentiviral

Expression

Mammalian

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AMBRA1-3xFLAG

Plasmid

#172605

Purpose

Expresses 3xFLAG-tagged AMBRA1 in mammalian cells

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGWB542

Plasmid

#74876

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB518

Plasmid

#74860

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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plRL58

Plasmid

#166886

Purpose

Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)

Depositor

Jeremy Rock

Publication

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pPROK AdoHcyase

Plasmid

#14683

Depositor

Michael Hershfield

Publication

Coulter-Karis et al Ann Hum Genet. 1989 May . 53(Pt 2):169-7

Insert

adenosyl-homocysteine hydrolase (AHCY Human)

Expression

Bacterial

Available since

May 1, 2007

Availability

Academic Institutions and Nonprofits only
pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Publication

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-DIO-EF1α-mKate2-biPAC

Plasmid

#169127

Purpose

AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

Depositor

Georg Nagel

Publication

Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

Insert

mKate2-biPAC

Use

AAV and Cre/Lox

Tags

mKate2

Expression

Mammalian

Mutation

codon optimized to mouse

Promoter

EF1a

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-rtTA

Plasmid

#163601

Purpose

Expresses rtTA in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rtTA

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR-p38KTRmCerulean3

Plasmid

#59149

Purpose

ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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