We narrowed to 12,150 results for: 112

1. pTn7CdrA-gfpC

   Plasmid

   #111616

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.10-Claudin-1

   Plasmid

   #46387

   Purpose

   Claudin-1 promoter reporter assay

   Depositor

   David Mu

   Article

   Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

   Insert

   CLDN1 Promoter (CLDN1 Human)
   

   Use

   Luciferase

   Promoter

   CLDN1 promoter

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pSAG1::CAS9-U6::sgUPRT

   Plasmid

   #54467

   Purpose

   Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.

   Depositor

   David Sibley

   Article

   Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

   Insert

   SAG1 5' UTR
   

   Use

   CRISPR; Toxoplasma expression

   Available Since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSD141B

   Plasmid

   #218139

   Purpose

   Lentiviral vector expressing evolved degron SD56 fused to eGFP with mCherry control.

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD56-eGFP-IRES2-mCherry
   

   Use

   Lentiviral

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-PASS

   Plasmid

   #193970

   Purpose

   Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

   Depositor

   Guangwei Du

   Article

   Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

   Insert

   PA biosenor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pV1382

   Plasmid

   #111436

   Purpose

   S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6-BbsI-GGsite-mCherry-Puro

   Plasmid

   #221232

   Purpose

   Cloning vector for sgRNA with mCherry and Puro

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_TRE_Ecad

   Plasmid

   #133805

   Purpose

   inducible expression of Ecad in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   Homo sapiens cadherin 1 (CDH1) (CDH1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0078 RESCUE

   Plasmid

   #130661

   Purpose

   pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   dRanCas13b-ADAR2dd (RESCUE)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWR76

    Plasmid

    #202776

    Purpose

    episomal fluorescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCherry
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid

    #185919

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR_SFFv_4D5-Low-CAR_RHL001

    Plasmid

    #164824

    Purpose

    Lentiviral vector for constitutive expression of AntiHER2 4D5-Low CAR

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV2-GCaMP6f-P2A-H2B-PAmCherry

    Plasmid

    #133419

    Purpose

    Fluorescent reporter for calcium imaging and photoactivation

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-G-GECO1.2

    Plasmid

    #32446

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.2
    

    Expression

    Mammalian

    Mutation

    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. UKNP1.18.1

    Plasmid

    #98190

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pV1326

    Plasmid

    #111435

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. UKNP1.9.1

    Plasmid

    #97374

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115211

    Purpose

    Expresses PsmCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PsmCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. phage ubc nls ha pcp gfp

    Plasmid

    #64539

    Purpose

    Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    PCP
    

    Use

    Lentiviral

    Tags

    EGFP, FactorXa site, HA, and NLS

    Expression

    Mammalian

    Promoter

    human ubiquitin C promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. PLX_307 GFP

    Plasmid

    #184492

    Purpose

    Lentivirus expression for GFP

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    GFP
    

    Use

    Lentiviral

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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