We narrowed to 65,546 results for: nin

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eGFP L202 gRNA

Plasmid

#119132

Purpose

gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

eGFP L202 gRNA

Use

CRISPR

Mutation

None

Promoter

U6

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-NRAS

Plasmid

#116767

Purpose

Lentiviral expression of NRAS

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NRAS (NRAS Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-UAS-Dendra2

Plasmid

#97145

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Dendra2

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab13-Q67L

Plasmid

#110502

Purpose

Constitutive Active

Depositor

Bernardo Mainou

Insert

Rab13-Q67L (RAB13 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q67L

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos16)GFP

Plasmid

#89249

Purpose

Expresses GFP with +16 theoretical charge in E.Coli

Depositor

David Liu

Article

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos16)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV1

Plasmid

#78633

Purpose

GESTALT V1 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT target V1

Use

Lentiviral

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ155

Plasmid

#69306

Purpose

Gateway entry vector with AteCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9D10A (Arabidopsis codon optimized)

Use

CRISPR

Mutation

D10A mutation; NLS fusion

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR FL CPAP RNAi resistant

Plasmid

#46390

Depositor

Pierre Gonczy

Article

Kitagawa et al J Cell Sci. 2011 Nov 15;124(Pt 22):3884-

Insert

CPAP RNAi resistant mutant (CENPJ Human)

Use

Gateway Cloning

Mutation

silent mutation that renders it resistant to siRN…

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only
NeonGreen-C1-ORP5

Plasmid

#220071

Purpose

overexpresses ORP5 with a neonGreen fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5 (OSBPL5 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSL4393 (pClpX_Eco)

Plasmid

#200907

Purpose

Expresses human codon-optimized E. coli ClpX from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

EcoClpX

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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SpyTag-sfGFP

Plasmid

#184226

Purpose

Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

SpyTag-sfGFP

Tags

His6, SpyTag, and TEV protease cleavage site

Expression

Bacterial

Promoter

T5

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (WT)

Plasmid

#169720

Purpose

Mammalian Expression of wild-type mouse SPOP

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pETNb-nALFA

Plasmid

#159988

Purpose

For the protein expression with ALFA tag at N-terminus in E. coli

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

Expression

Bacterial

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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elavl3:SomaGCaMP7f

Plasmid

#158760

Purpose

Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP7

Use

Synthetic Biology

Promoter

HuC promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe-EGFP-Hu_Plk1(WT)

Plasmid

#136323

Purpose

Retroviral expression of human Plk1-WT with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Insert

PLK1 (PLK1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_EGFP-LAMA-R59 (for ALFA-tag)

Plasmid

#136627

Purpose

Plasmid encoding the bacterial expression of LAMA-R59 for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-LAMA-R59

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC1A4

Plasmid

#131962

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC1A4 (SLC1A4 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMVA

Plasmid

#121149

Purpose

This plamid was used for improving the production of DMAPP (IPP) in E.coli

Depositor

Minjuan Xu

Article

He et al ACS Synth Biol. 2018 Sep 21;7(9):2094-21

Insert

MVA Pathway (ERG12, ERG8, MVD1, IDI, MvaE, MvaS )

Use

Synthetic Biology

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oHept

Plasmid

#124671

Purpose

Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHept

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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YA1531: pAAV_CAG-FLEX-QuasAr3

Plasmid

#107701

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr3

Use

AAV

Expression

Mammalian

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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