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pLAP-MIS12-KARD-3A

Plasmid

#114059

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

MIS12-KARD-3A (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

BUBR1-S670A, S676A and T680A

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/666E

Plasmid

#105313

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/666E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP KInd2 S159E

Plasmid

#105311

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/666A

Plasmid

#105309

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/666A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S666A

Plasmid

#105308

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S666A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP KInd2 S159A

Plasmid

#105306

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159A

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CIP2Aprom204bp-pGL4.10Luc

Plasmid

#60874

Purpose

Luc reporter driven by 204 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

204 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only
CIP2Aprom335bp-pGL4.10Luc

Plasmid

#60872

Purpose

Luc reporter driven by 335 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

335 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only
CIP2Aprom392bp-pGL4.10Luc

Plasmid

#60871

Purpose

Luc reporter driven by 392 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

392 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only
CIP2Aprom417bp-pGL4.10Luc

Plasmid

#60870

Purpose

Luc reporter driven by 417 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

417 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only
CIP2Aprom865bp-pGL4.10Luc

Plasmid

#60869

Purpose

Luc reporter driven by 865 bp of the CIP2A promoter

Depositor

Jukka Westermarck

Article

Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

Insert

865 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)

Use

Luciferase; Promoterless

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only
pCAG-HDAC4*

Plasmid

#71402

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4* (HDAC4 Human)

Expression

Mammalian

Mutation

After aa126, insertion leads to reading frame shi…

Promoter

CAG

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT TO myc Separase delta 55/Non-Cleavable

Plasmid

#59827

Purpose

Allows the integration of myc Separase delta 55/Non-Cleavable in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

delta 55/Non-Cleavable (E1483R, R1486E, E1503R, R…

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-ACIN1-sgRNA1

Plasmid

#165925

Purpose

CRISPR-mediated gene perturbation (knock-out) of ACIN1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

ACIN1 (ACIN1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-ACIN1-sgRNA1

Plasmid

#165926

Purpose

CRISPR-mediated gene perturbation (knock-out) of ACIN1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

ACIN1 (ACIN1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-HK2-sgRNA1

Plasmid

#165929

Purpose

CRISPR-mediated gene perturbation (knock-out) of HK2

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

HK2 (HK2 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-HK2-sgRNA2

Plasmid

#165930

Purpose

CRISPR-mediated gene perturbation (knock-out) of HK2

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

HK2 (HK2 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-LUC7L2-sgRNA1

Plasmid

#165935

Purpose

CRISPR-mediated gene perturbation (knock-out) of LUC7L2

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

LUC7L2 (LUC7L2 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-LUC7L2-sgRNA2

Plasmid

#165936

Purpose

CRISPR-mediated gene perturbation (knock-out) of LUC7L2

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

LUC7L2 (LUC7L2 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-puro-LUC7L2-3xFLAG

Plasmid

#165952

Purpose

Overexpression of LUC7L2.

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

LUC7L2 (LUC7L2 Human)

Use

Lentiviral

Tags

3x-FLAG

Promoter

EF1-alpha

Availability

Academic Institutions and Nonprofits only

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