We narrowed to 17,271 results for: POR

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pSC-mCh-P2A-ST-EGFP-CAAX

Plasmid

#207636

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX

Expression

Mammalian

Mutation

Amber stop codon at SpyCatcher’s critical lysine

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-WWE-Linker-eGFP

Plasmid

#176072

Purpose

EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

WWE

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Y951F-VEGFR2-mCherry

Plasmid

#139607

Purpose

Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-alpha-E-Catenin 3xFP4 (895)

Plasmid

#67936

Purpose

Mammalian expression of alpha E catenin

Depositor

Alpha Yap

Article

Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

Insert

alpha E catenin (Ctnna1 Mouse)

Tags

3xFP4 and EGFP

Expression

Mammalian

Mutation

N-terminal domain

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
h-Plekhm1_FLAG-pcDNA 3.1

Plasmid

#73593

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-PBZC-Linker-eGFP

Plasmid

#176070

Purpose

EGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PBZ-C

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-PBM-Linker-eGFP

Plasmid

#176069

Purpose

EGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

PBM

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-KPNB1

Plasmid

#192301

Purpose

Gateway entry vector for an inducible 3xFLAG-KPNB1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNB1 (KPNB1 Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mRuby3-6xFLAG

Plasmid

#161613

Purpose

Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

mRuby3-6xFLAG

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human synapsin 1 promoter

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955

Plasmid

#134613

Purpose

Mammalian expression of rat KIF5C 378-955

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5C (Kif5c Rat)

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 377 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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FKBP-beta1(617-949)-GFP

Plasmid

#100732

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP1B1 (AP1B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-eGFP_mito

Plasmid

#221414

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

eGFP_mito

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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PGK ARF6(Q67L)-CFP

Plasmid

#200428

Purpose

Mammalian expression of Q67L mutation in ARF6

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

ARF6 (ARF6 Human)

Tags

CFP

Expression

Mammalian

Mutation

Q67L

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGAPTrap TALEN 2

Plasmid

#83369

Purpose

Partner to pGAPTrap-TALEN1, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN2

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3

Plasmid

#170175

Purpose

Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME2f-ST-P2A-NLS-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_hCOL4A5-Nluc_R1683X

Plasmid

#198124

Purpose

Express hCOL4A5_R1683X in mammalian cells

Depositor

Jeffrey Miner

Article

Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

Insert

COL4A5 (COL4A5 Human)

Tags

Nluc

Expression

Mammalian

Mutation

R1683X

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-TALEN1

Plasmid

#83368

Purpose

Partner to pGAPTrap-TALEN2, for enhanced gene targeting of the human GAPDH locus using GAPTrap vectors

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

GAPDH_TALEN1

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv1_SDHA

Plasmid

#177980

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting SDHA (SDHA Human)

Use

Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR

Plasmid

#61624

Purpose

FRET-based biosensor for monitoring Akt activity

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR (AKT1 Budding Yeast, Synthetic, Human)

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-UAP56

Plasmid

#157661

Purpose

Expresses UAP56 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Spliceosome RNA helicase DDX39B (DDX39B Human)

Tags

GST tag

Expression

Bacterial

Mutation

deleted amino acids 1-43

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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