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413 pSG5L HA RB

Plasmid

#10720

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB (RB1 Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry

Plasmid

#159655

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pICE-HA-MRE11-WT

Plasmid

#82033

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Sébastien Britton, Patrick Calsou

Article

Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

Insert

MRE11 (MRE11 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV-tet

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.GCaMP6f.WPRE.SV40

Plasmid

#100835

Purpose

AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV2, AAV5, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_rDA1m

Plasmid

#140556

Purpose

Expresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

Red fluorescent DA sensor GRAB_rDA1m (medium affinity)

Use

AAV

Promoter

hSyn

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9

Plasmid

#231910

Purpose

For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA

Plasmid

#84468

Purpose

pAAV-CRAM-tdTomato

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

tdTomato

Use

AAV

Mutation

T230I (please see depositor comments below)

Promoter

TRE

Available Since

Dec. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ChR2-WPREpA

Plasmid

#84471

Purpose

pAAV-RAM-ChR2

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Has Service

AAV8

Insert

Channelrhodopsin 2

Use

AAV

Tags

EYFP

Mutation

H134R

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-ArchT-WPREpA

Plasmid

#84472

Purpose

pAAV-RAM-ArchT

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Insert

ArchT

Use

AAV

Tags

EGFP

Promoter

TRE

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204341

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBBR1.cas9-NT

Plasmid

#197871

Purpose

Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

Depositor

Baojun Wang

Article

Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

Inserts

trancCRNA
Cas9
crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSMART Tmem192-3X HA (targeting vector for genomic tagging)

Plasmid

#175777

Purpose

pSMART Tmem192-3X HA (targeting vector for genomic tagging)

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TMEM192 (TMEM192 Human)

Use

CRISPR

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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AnkyrinG-190-GFP

Plasmid

#31059

Depositor

Vann Bennett

Article

Mohler et al J Biol Chem. 2002 Mar 22. 277(12):10599-

Insert

Ankyrin G (Ank3 Rat)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i1 ONE-GO

Plasmid

#189735

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i1 ONE-GO
GNAI1 (Gnai1 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

Plasmid

#84467

Purpose

Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Promoter

pRAM and TRE

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-C2-SNAP

Plasmid

#208769

Purpose

To express C2-SNAP in bacteria

Depositor

Urte Neniskyte

Article

Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

Insert

C2-SNAP (Mfge8 Mouse)

Tags

SNAP-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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AV4-EF1-NMNAT2

Plasmid

#124970

Purpose

Express NMNAT2

Depositor

Kazuhiro Oka

Article

Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

Insert

NMNAT2, GFP (NMNAT2 Human)

Use

AAV

Promoter

EF1-alpha

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pWPXL-Sox4

Plasmid

#36984

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox4 (SOX4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 13, 2012

Availability

Academic Institutions and Nonprofits only

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pRibo-APEX2m

Plasmid

#176842

Purpose

Expression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)

Depositor

Jessica Seeliger

Article

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-APEX2m

Expression

Bacterial

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINS-USP21 (USP, aa 196-565)

Plasmid

#61585

Purpose

Expresses human USP21 (USP domain) in E. coli.

Depositor

David Komander

Article

Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

Insert

USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)

Tags

His6-SUMO

Expression

Bacterial

Mutation

Deleted aa 1-195.

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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