We narrowed to 9,182 results for: Pol

1. pICH41414

   Plasmid

   #50337

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   3'UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC1821_pCR8-SMN2-DN-RG6-SA

   Plasmid

   #118655

   Purpose

   Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   SMN2-DN-RG6-SA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICH71431

   Plasmid

   #50344

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   3'UTR, polyadenylation signal/terminator, ATPase (S. lycopersicum )
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. 438-GST

   Plasmid

   #97092

   Purpose

   GST-tev-yORF

   Depositor

   Chris Jeans

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   GST tag and TEV cleavage site

   Expression

   Insect

   Promoter

   Polyhedrin promoter

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pL2-G(P)10G

   Plasmid

   #137105

   Purpose

   iFLinkC Linker cloning

   Depositor

   Viktor Stein

   Article

   Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

   Insert

   G(P)10G
   

   Use

   Synthetic Biology

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pL2-G(TP)4TG

   Plasmid

   #137101

   Purpose

   iFLinkC Linker cloning

   Depositor

   Viktor Stein

   Article

   Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

   Insert

   G(TP)4TG
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. tet-pLKO.puro_shHuR CDS

   Plasmid

   #110411

   Purpose

   TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAVL1 (HuR)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1/TO (RNA PolIII)

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUG-57

   Plasmid

   #104714

   Purpose

   Cloning Module

   Depositor

   Marco Trujillo

   Article

   Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

   Insert

   Ub10 (UBQ10 Mustard Weed)
   

   Use

   Synthetic Biology

   Available Since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MBP-3C-hNorrin(33-133)-1D4_pAcGP67a

   Plasmid

   #216384

   Purpose

   Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Norrin (NDP Human)
   

   Use

   Baculovirus

   Tags

   GP64 signal sequence-MBP-3C and Rho1D4

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLfa-PUb-EGFP-3'UTR#5

    Plasmid

    #176337

    Purpose

    The synthetic 3'UTR#5 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

    Depositor

    Zach Adelman

    Article

    Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

    Insert

    3'UTR#5
    

    Expression

    Insect

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. tet-pLKO.puro_shGAC

    Plasmid

    #110423

    Purpose

    shGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICH41432

    Plasmid

    #50343

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, ocs (A. tumefaciens)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDB023f

    Plasmid

    #99217

    Purpose

    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] T7RNAP(S12*,S203*)
    

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. KCNK2

    Plasmid

    #73257

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KCNK2 (KCNK2 Human)
    

    Tags

    TEV-His10-FLAG

    Expression

    Insect

    Mutation

    userdefined:Full_Length 1411

    Promoter

    Polyhedrin

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. CDK12

    Plasmid

    #73258

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CDK12 (CDK12 Human)
    

    Tags

    His-6-TEV

    Expression

    Insect

    Mutation

    pfam00069: Pkinase 727-1020

    Promoter

    Polyhedrin

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pUC57-Rluc-BoxB-A114-N40

    Plasmid

    #50562

    Purpose

    Template DNA for in vitro transcription using T7 polymerase

    Depositor

    Yukihide Tomari

    Article

    Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

    Insert

    Luciferase-BoxB-A114-N40
    

    Use

    Cloning vector

    Promoter

    lac

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCY 3080-01

    Plasmid

    #36226

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    yEmCitrine
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-yEmCitrine-kanMX

    Available Since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRL2960

    Plasmid

    #70694

    Purpose

    pDU1-E. coli shuttle vector (Km-r) with extended polylinker

    Depositor

    Peter Wolk

    Article

    Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

    Type

    Empty backbone

    Use

    Bacterial shuttle vector

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCY 3030-02

    Plasmid

    #36215

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    yEmCFP
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-yEmCFP-hphMX4

    Available Since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCY 3110-01

    Plasmid

    #36236

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    mEos2
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-mEos2-kanMX

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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