We narrowed to 14,954 results for: flag

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pFETCh_RAD21

Plasmid

#64056

Purpose

Homology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RAD21 Homology Arms (RAD21 Human)

Tags

3X Flag P2A Neo

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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GP734

Plasmid

#164527

Purpose

Expresses Flag-Ub

Depositor

Gregory Pazour

Publication

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin

Use

Lentiviral

Tags

FLAG and Ub

Mutation

WT

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

Plasmid

#214995

Purpose

Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

Depositor

Wade Harper

Publication

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag

Plasmid

#191537

Purpose

Overexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NLS-ddMisCas13b-SNAPf-NLS-3xFlag

Expression

Mammalian

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pKS023 - pCAGGS-3XFLAG-mKate2-Pds5b-bpA

Plasmid

#156442

Purpose

For transient expression of mouse PDS5B tagged with mKate2

Depositor

Elphege Nora

Publication

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5B (Pds5b Mouse)

Expression

Mammalian

Mutation

none

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pKS016 - pCAGGS-3XFLAG-mKate2-Pds5a-bpA

Plasmid

#156439

Purpose

For transient expression of mouse PDS5A tagged with mKate2

Depositor

Elphege Nora

Publication

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5A (Pds5a Mouse)

Expression

Mammalian

Mutation

none

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_FLAG3

Plasmid

#223896

Purpose

4G-cloning donor for C-terminal FLAG3 tag

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

FLAG3

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGS_N_3xFLAG

Plasmid

#101654

Purpose

Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Publication

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, 3xFLAG tag

Tags

3xFLAG tag

Expression

Insect

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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N-SF-TAP-DEST-TLK2-D592V

Plasmid

#218835

Purpose

Expresses N-terminal Strep-FLAG-tagged human TLK2 mutant D592V (kinase dead).

Depositor

Travis Stracker

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TLK2 Q592V (kinase dead) (TLK2 Human)

Tags

FLAG-STREP

Expression

Mammalian

Mutation

Q592V

Promoter

CMV

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCDZ2FE_Rev3_1873-2025_4PA

Plasmid

#105673

Purpose

Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein with mutations abolishing Rev7 binding

Depositor

Thanos Halazonetis

Insert

Rev3L (REV3L Human)

Tags

2X Flag tag

Expression

Mammalian

Mutation

Mutations: P1880A, P1885A, P1996A, P2001A

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-Y530F

Plasmid

#140319

Purpose

Expresses Flag-tagged human Src-Y530F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Publication

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X Flag

Expression

Mammalian

Mutation

Y530F

Promoter

CMV

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLexM-SLC7A11

Plasmid

#197993

Purpose

Expresses SLC7A11 with an N-terminal FLAG tag in mammalian cells

Depositor

Simon Newstead

Publication

Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

Insert

SLC7A11 (SLC7A11 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225062

Purpose

Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Publication

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM

Plasmid

#225061

Purpose

Expression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Publication

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-nsp3-c3xFLAG

Plasmid

#215696

Purpose

Produces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Publication

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CHIKV nsp3

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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CAGGS-EGFP-NLS-3XFLAG-V5

Plasmid

#196090

Purpose

Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

Depositor

Mauro Calabrese

Publication

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP

Tags

C-terminal 3xFLAG and C-terminal V5

Expression

Bacterial and Mammalian

Promoter

CAGGs

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHR RPS27A-3xFlag_IRES-GFP

Plasmid

#198390

Purpose

RPS27A (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Publication

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-KPNA2 zeo

Plasmid

#192330

Purpose

Lentiviral expression vector for an inducible 3xFLAG-KPNA2

Depositor

Kevin Janes

Publication

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA2 (KPNA2 Human)

Use

Lentiviral

Tags

3xFLAG

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-MCS-BioID2-3xFLAG

Plasmid

#186812

Purpose

CAG promoter-driven expression of BioID2-fused protein (C terminus)

Depositor

Masahito Ikawa

Publication

Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

Type

Empty backbone

Tags

BioID2-3xFLAG

Expression

Mammalian

Promoter

CAG promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMRX-IP-3xFLAG-NCOA4 (I56E)

Plasmid

#192813

Purpose

Stable expression of 3xFLAH-NCOA4 (I56E mutant) in mammalian cells

Depositor

Noboru Mizushima

Publication

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

I56E mutation in the N-terminal domain

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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