We narrowed to 7,402 results for: Lif;

1. pcDNA 5/FR/TO-Ubl4A K48R

   Plasmid

   #61811

   Purpose

   express C-terminal Ubl4A K48R, for making the stable cell line

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   UBL4A (UBL4A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K48R

   Promoter

   CMV

   Available since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pPRISM-QF2-he1.1:YFP

   Plasmid

   #184819

   Purpose

   Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   QF2-pA-he1.1:YFP
   

   Use

   CRISPR; Backbone for cloning of homology arms (fo…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPRISM-QF2-he1.1:YFP-rln3a-HA

   Plasmid

   #184820

   Purpose

   Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   rln3a-HA
   

   Use

   CRISPR; For integration of qf2 by homology-direct…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

   Plasmid

   #163925

   Purpose

   Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
   

   Use

   Cre/Lox

   Tags

   tagBFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pProEx-Htb-cytATL2-6Gly-sfGFP

   Plasmid

   #124065

   Purpose

   E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   cytATL-sfGFP
   

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1-OSF-TRIMCyp (owl monkey)

   Plasmid

   #79035

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5Cyp (owl monkey)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-mCherry-Emi1_ARHGAP5 UTRs

   Plasmid

   #67927

   Purpose

   Expresses mCherry-Emi1 with ARHGAP5 UTRs in mammalian cells

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

   Insert

   mCherry-Emi1 (FBXO5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW4.28

   Plasmid

   #62878

   Purpose

   Bacterial expression of MBP-MEG-3 T541A S582A T605A

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   MBP-MEG-3 T541A S582A T605A
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   T541A S582A T605A

   Promoter

   Available since

   March 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJW3.31

   Plasmid

   #62865

   Purpose

   Yeast two-hybrid bridge vector expressing PAA-1

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   PAA-1/Y3H
   

   Use

   Two-hybrid bridge vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available since

   March 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Ubl4A K48R

    Plasmid

    #61809

    Purpose

    expresses mutated Ubl4A with K48R, with a C-terminal FLAG tag

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    UBL4A (UBL4A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K48R

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only