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pAAV-EF1a-DIO-mGreenLantern

Plasmid

#164468

Purpose

Cre-dependent EF1a-driven expression of mGreenLantern in animals using AAV

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

AAV

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

EF1a

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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IY704: pMVP (L1-R5) EF1a promoter

Plasmid

#121693

Purpose

pMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC293 - pAAV EF1a V5-synuclein (WT)

Plasmid

#60057

Purpose

An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

alpha-synuclein (SNCA human, Human)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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hAAVS1-gs4_EF1a-Cas9-2A-GFP

Plasmid

#118414

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA AAVS1 (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

Plasmid

#229800

Purpose

Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mGreenLantern; mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

hEF1a and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas3

Plasmid

#178881

Purpose

Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas3c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLENTI EF1alpha FLAG/HA-PALI1

Plasmid

#114445

Purpose

Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human PALI1 coding sequence

Depositor

Adrian Bracken

Article

Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

Insert

PALI1 (LCOR Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-E690Kmut_ABCA3-GFP

Plasmid

#188549

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3:GFP (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Mutation

amino acid E690->K690 (ABCA3 gene)

Promoter

EF1aL

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5 + PGK-blast

Plasmid

#167818

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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SM470_EF1A-dEnAsCas12a-T2A-EGFP

Plasmid

#244222

Purpose

Mammalian expression of dEnAsCas12a with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-scFv-VP64_Blast

Plasmid

#192655

Purpose

3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

scFv-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pOttc556 - pAAV EF1a iCre

Plasmid

#210416

Purpose

AAV viral vector packaging plasmid that expresses "improved Cre recombinase"

Depositor

Brandon Harvey, Christopher Richie

Insert

iCre

Use

AAV

Promoter

EF1a

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-ER-KLF2-T2A-mCHERRY

Plasmid

#224297

Purpose

Expresses estrogen receptor linked KLF2 and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

Estrogen Receptor linked KLF2 T2A mCherry (Klf2 Mouse)

Use

Retroviral

Tags

Estrogen Receptor

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-HTB

Plasmid

#44187

Depositor

Edward Callaway

Insert

Histone tagged GFP, T2A-TVA-E2A-B19G

Use

AAV; Adeno-associated vector

Tags

GFP

Promoter

EF1a

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only
pEF1a FLAG-CHMP2B-d55-96

Plasmid

#232013

Purpose

Expression of FLAG-tagged CHMP2B with the second alpha helix deleted.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Δ55-96

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-KLF2-T2A-mCHERRY

Plasmid

#224295

Purpose

Expresses mouse KLF2 and mCherry under EF1a promoter

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

KLF2 T2A mCherry (Klf2 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLv-EF1a-Parkin-A92mKO2

Plasmid

#213559

Purpose

Lentiviral vector for stable expression of Parkin, internally tagged with mKO2

Depositor

Steven Altschuler, Lani Wu

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAF0466 Ef1a-Nm60_attB acceptor

Plasmid

#193476

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Nm60_attB

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO-mCherryP2ACre

Plasmid

#161773

Purpose

Flp-Dependent mCherry and Cre expression

Depositor

Baoji Xu

Article

An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

Insert

mCherry-P2A-Cre

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5-ires-blast

Plasmid

#167822

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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