-
Plasmid#34576DepositorInsertASS1 (Ass1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-RAD52-WT
Plasmid#235676PurposeExpresses HA-tagged wild type human RAD52 in mammalian cells.DepositorInsertRAD52 (RAD52 Human)
UseTagsHAExpressionMammalianMutationwild typePromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 CMV NAPA-A BGH
Plasmid#92277PurposeFRET probe; extracellular membrane tethered eGFPDepositorInsertNeuron-astrocyte proximity assay - Astrocyte
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras light chain
Plasmid#216294PurposeExpresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras FabDepositorInsertTras light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Pert Light chain
Plasmid#216310PurposeExpresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert FabDepositorInsertPert Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
FmnI IS04 cDNA/pcDNA3
Plasmid#19321DepositorInsertFmnI Iso4 cDNA (Fmn1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-39S Light chain
Plasmid#216297PurposeExpresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S FabDepositorInsert39S Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MF3958 Light chain
Plasmid#216304PurposeExpresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 FabDepositorInsertMF3958 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3-YFP
Plasmid#50484Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsYFPExpressionMammalianMutationYFP fusion in M3-M4 loop (after residue P379). O…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA D122A pcDNA3
Plasmid#206120Purposeexpression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1DepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationD122APromoterCMVAvailable sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 EIVA (E1707A) pcDNA3
Plasmid#206125Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation bindingDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationE1707APromoterCMVAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA EIA (E320A) pcDNA3
Plasmid#206124Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation bindingDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationE320APromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3-CFP
Plasmid#50485Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsCFPExpressionMammalianMutationCFP fusion in M3-M4 loop (after residue P379). O…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA Arf1 DN T31N
Plasmid#10833DepositorInsertArf1 T31N (ARF1 Human)
UseTagsHAExpressionMammalianMutationT31N. Defective in GTP binding. Dominant negati…PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 myc card9
Plasmid#16253DepositorInsertcard9 (CARD9 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag C/EBPa
Plasmid#66978PurposeExpression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of proteinDepositorInsertC/EBP alpha (Cebpa Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf5(T31N)-HA
Plasmid#79429PurposeExpresses C-terminally HA-tagged Arf5(T31N) in mammalian cellsDepositorInsertArf5 (ARF5 Human)
UseTagsHAExpressionMammalianMutationchanged Threonine 31 to AsparaginePromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf5(Q71L)-HA
Plasmid#79428PurposeExpresses C-terminally HA-tagged Arf5(Q71L) in mammalian cellsDepositorInsertArf5 (ARF5 Human)
UseTagsHAExpressionMammalianMutationchanged Glutamine 71 to LeucinePromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-TORCAR
Plasmid#64929PurposeLysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.DepositorInsertLAMP1-TORCAR (LAMP1 Synthetic, Human)
UseTagsCerulean, Full-length LAMP1, and YPetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS pcDNA3
Plasmid#206057Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain IIDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only