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pMVV108

Plasmid

#215338

Purpose

Expresses sfGFP under induction with the Salicylic Acid Marionette Cassette (NahRAM + PSalTTC-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PSalTTC

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-IETD-NanoLuc

Plasmid

#98288

Purpose

caspase-8 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-P2A-3xFLAG-hEEA1 HDR template

Plasmid

#214989

Purpose

Endogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

CRISPR

Tags

3xFLAG

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV207

Plasmid

#215343

Purpose

gRNA expression for S. stutzeri nifL deletion on an RSF1010 backbone

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

S. stutzeri nifL gRNA

Use

CRISPR and Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

J23119

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV205

Plasmid

#215342

Purpose

Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backbone

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-P2A-3xFLAG-msEEA1 HDR template

Plasmid

#214990

Purpose

Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (Eea1 Mouse)

Use

CRISPR

Tags

3xFLAG

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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SNAPtag-LacI

Plasmid

#183922

Purpose

Lac repressor labeled with SNAPtag; can be used to label lacO sites

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

SNAPtag-LacI (lacI Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pICH-roGFP2-GPXC71S

Plasmid

#191698

Purpose

Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a control roGFP2 fusion not responsive to peroxides used alongside plasmids 191680 and 191677

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

redox(ro)GFP2-GPXC71S

Expression

Plant

Mutation

Peroxidatic Cysteine 71 replaced with Serine

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTK332

Plasmid

#59573

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-mTIP49a

Plasmid

#15357

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

TATA binding protein interacting protein 49 kDa (Ruvbl1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pMVV098

Plasmid

#215304

Purpose

Expresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PPhIF

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pac

Plasmid

#126957

Purpose

Promoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTK333

Plasmid

#59574

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FBL6 delta6

Plasmid

#19925

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

FBL6 delta 6 (FBXL6 Human)

Expression

Mammalian

Mutation

delta 6

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only
MO70 Orai1 N223A

Plasmid

#21663

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai1 (ORAI1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

N223A

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
FUW-TetO-Gata1

Plasmid

#41085

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Gata1 (Gata1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO + minimal CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-Amida

Plasmid

#15362

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

TCF3 (E2A) fusion partner (TFPT Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pEBG-TFII-I-delta N90

Plasmid

#22165

Depositor

Ananda Roy

Article

Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Mutation

The first 90 aa of TFII-I are deleted

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pIVEX2.3 TEM-8

Plasmid

#16563

Depositor

Bert Vogelstein

Article

Carson-Walter et al Cancer Res. 2001 Sep 15. 61(18):6649-55.

Insert

TEM8 (ANTXR1 Human)

Expression

Bacterial

Mutation

Extracellular region

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pNRB143

Plasmid

#36452

Depositor

Douglas Bishop

Article

Bishop et al Genes Cells. 1999 Aug;4(8):425-44.

Insert

RAD54 (RAD54 Budding Yeast)

Expression

Yeast

Promoter

T7

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pEBB-BT-Ubiquitin

Plasmid

#36098

Depositor

Ezra Burstein

Article

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Insert

Ubiquitin (RPS27A Human)

Tags

TB-tag

Expression

Mammalian

Promoter

EF1a

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

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pJUMP27ts[mCherry]-1A(lacZ)

Plasmid

#126980

Purpose

Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Linker-hSyn::mCherry.3xFLAG-WPRE

Plasmid

#120391

Purpose

pAAV plasmid expressing mCherry.3XFLAG under the hSyn promoter with a Linker containing BstBI, BspQI and SacI sites upstream of the hSyn promoter

Depositor

Florian Freudenberg

Article

Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

Type

Empty backbone

Use

AAV

Tags

mCherry.3XFLAG

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP18-pheS_NOC

Plasmid

#127031

Purpose

Basic Part NOC- Complete ORF; PheS; counter-selection marker; mutant phenylalanine tRNA synthetase.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part PheS_NOC

Use

Synthetic Biology

Expression

Bacterial

Mutation

pheS A294G promiscuous mutation

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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MO70 Orai1 E190Q

Plasmid

#21662

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai1 (ORAI1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

E190Q

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
acta 2

Plasmid

#78711

Purpose

zebrafish acta2 3-UTR–specific probe

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

acta2 (acta2 Zebrafish)

Expression

Bacterial

Mutation

contains bp 1,243–1,591

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
plentiCRISPR-sgMDH1

Plasmid

#72875

Purpose

The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgMDH1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
pJUMP18-B0034-MV_RN

Plasmid

#127017

Purpose

Basic Part RN- RBS+N-terminus; pET vector Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0034m-MV_RN

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc Caronte (CT#612)

Plasmid

#13861

Depositor

Cliff Tabin

Article

Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

Insert

Caronte (CER1 Chicken)

Tags

myc

Expression

Mammalian

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pFLAG Caronte (CT#304)

Plasmid

#13860

Depositor

Cliff Tabin

Article

Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

Insert

Caronte (CER1 Chicken)

Tags

flag

Expression

Mammalian

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only

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