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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,049 results for: golden gate

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Showing: 1901 - 1950 of 5049 results

  18. pRDA_835

    Plasmid
    #245327
    Purpose
    Cas9 CRISPRko positive control guide; targets CD59
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD59 (CD59 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245327-plasmid-map-sequence-id-488049

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  22. POWV strain LB NS2B-NS3 protease fusion with non cleavable linker

    Plasmid
    #228654
    Purpose
    Bacterial expression of the fusion of the NS2B-NS3 protease from Powassan virus for biochemical assay
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Powassan virus portion of NS2B protease fused to NS3
    Tags
    His-Twin Strep-Tev
    Expression
    Bacterial
    Mutation
    Alanine mutant to stop self-cleaving ..VEREEA*AL.…
    Available Since
    Feb. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228654-plasmid-map-sequence-id-461566

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  25. pAIP4_3KT9

    Plasmid
    #234192
    Purpose
    Heterologous protein expression of aprataxin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3KT9 (APTX Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234192-plasmid-map-sequence-id-464157

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  34. pBTK1212

    Plasmid
    #251993
    Purpose
    Double-stranded RNA biomolecular fluorescence complementation sensor for bacteria, induced by arabinose. Variant of pDS-BiFC7 with AmpR.
    Depositor
    Jeffrey Barrick
    Article
    Navarro-Escalante et al bioRxiv 2026.01.18.700149
    Insert
    Split mVenus (YFP) fused to mutant B2 dsRNA-binding domains (RBDs)
    Use
    Biosensor
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Feb. 25, 2026
    Availability
    Academic Institutions and Nonprofits only
    251993-plasmid-map-sequence-id-500785

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  49. pAIP4_2AWG

    Plasmid
    #234222
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP8 (PPIase FKBP8) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2AWG (FKBP8 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234222-plasmid-map-sequence-id-464187

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  50. pAIP4_1FL0

    Plasmid
    #234216
    Purpose
    Heterologous protein expression of aminoacyl tRNA synthase complex-interacting multifunctional protein 1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1FL0 (AIMP1 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234216-plasmid-map-sequence-id-464181

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Showing: 1901 - 1950 of 5049 results