We narrowed to 2,481 results for: D10
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pcDNA3-Flag-Tet1 CD Mut
Plasmid#72218PurposeExpress flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTen-Eleven Translocation 1 (Tet1 Mouse)
TagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain (cata…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpRYc-P2A-EGFP
Plasmid#208336PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYcDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationSc++ (1-1119), SpRY (1111-1368), D10APromoterCAGAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME464
Plasmid#238068PurposeExpressed ME-ABE ABE7.10-HRHSK with SpRYCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTadA*7.10(L36H/L51R/Y123H/C146S/N157K)-m_SpRYnCas9(D10A)
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationTadA modifications include L36H, L51R, Y123H, C14…Available sinceJan. 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pMEH31 Lamp2b HA
Plasmid#168208PurposeMammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLamp2b (LAMP2 Human)
TagsHAExpressionMammalianPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCBEclos-cbei2385-g2-opt
Plasmid#118215PurposeCRISPR-mediated base editing in ClostridiumDepositorInsertApobec1, Casp nickase, UG1-sgRNA
ExpressionBacterialPromoterPtmAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9n-MS2-BB_BbsI
Plasmid#164804PurposeREDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFPDepositorTypeEmpty backboneExpressionMammalianAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnCas9-miniSdd7-PBE
Plasmid#204859PurposeFor cytosine base editing using miniSdd7 in monocotyledon plants (transient transformation)DepositorInsertminiSdd7-nSpCas9(D10A)-UGI
UseCRISPRExpressionPlantMutationD10A in SpCas9PromoterZmUbi-1Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-miniTurbo
Plasmid#219822PurposeEF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9
UseCRISPRTagsminiTurboExpressionMammalianMutationD10A/H841APromoterEF1aAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo
Plasmid#102885PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDDdCas9VP192-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
A3Ai-Cas9n-UGI-NLS
Plasmid#109425PurposeExpresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
UseCRISPRExpressionMammalianMutationInsertion of an L1 intron into the coding sequenc…PromoterCMVAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRS0035
Plasmid#131125PurposeMammalian Target-AID expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnCas9-PmCDA1-UGI
UseCRISPRTagsPmCDA1 and UGIExpressionMammalianMutationD10A in SpCas9PromoterCMV promoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gGBEv6.3-SpG
Plasmid#202630Purposevector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnSpG(D10A)-MPGv6.3
ExpressionMammalianMutationSpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…PromoterhU6, EF1a, CBhAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-ABE-Cas9n-Act3.0
Plasmid#178956PurposeIt consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.DepositorInsertABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRExpressionPlantAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
gag-TadCBEd
Plasmid#225144PurposePackaging plasmid for TadCBEd in v3 PE-eVLP architectureDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMMLVgag-TadCBEd
ExpressionMammalianAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHA-ΔN335-Kin1A
Plasmid#31606DepositorInsertΔN335 kinesin-1A (Kif5a Mouse)
TagsHA-tagExpressionMammalianMutationdeleted amino acids 1-335PromoterCMVAvailable sinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)
Plasmid#185913PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337RPromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo
Plasmid#102889PurposePiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDDdCas9VP192-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Mammalian_SpCas9_Target-AID2S
Plasmid#188648PurposeExpresses SpCas9_Target-AID2S in mammalian cellsDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnCas9-AID2S-UGI
TagsUGIExpressionMammalianMutationD10A for SpCas9PromoterpCMVAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
gCBEv2
Plasmid#220617Purposevector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthUNG2-nSpCas9
ExpressionMammalianMutationhUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)PromoterhU6, EF1aAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only
