We narrowed to 1,212 results for: Ncl

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KG#471

Plasmid

#110935

Purpose

Expresses proteins in a subset of neurons in C. elegans, including isolated expression in the DA9 cholinergic motor neuron in the tail

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2015 Sep;201(1):91-116. doi: 1

Inserts

mig-13 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLY133

Plasmid

#130961

Purpose

Engineered sgRNA-LEB4 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB4

Use

Synthetic Biology

Expression

Bacterial

Promoter

Anderson promoter J23100

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLY86

Plasmid

#130949

Purpose

Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA2-WTmis2

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLY132

Plasmid

#130960

Purpose

Engineered sgRNA-LEB3 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB3

Use

Synthetic Biology

Expression

Bacterial

Promoter

Anderson promoter J23100

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLY130

Plasmid

#130952

Purpose

Engineered sgRNA-LEB1 generator including Anderson promoter J23100 for golden gate assembly

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEB1

Use

Synthetic Biology

Expression

Bacterial

Promoter

Anderson promoter J23100

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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R2Ct (1-520)

Plasmid

#115649

Purpose

expression of truncated Rag2 including the C-terminal region

Depositor

Martin Gellert

Article

Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

Insert

Rag2 (1-520) (Rag2 Mouse)

Tags

8XHis-MBP-PreScission

Expression

Mammalian

Mutation

truncated Rag2 (aa 1-520)

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pES1004

Plasmid

#21790

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Kss1-as2-trunc (KSS1 Budding Yeast)

Expression

Yeast

Mutation

deleted bases 1-14 from open reading frame E94A …

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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p075 pCS xTRPS1 delN delC119 (M)

Plasmid

#11045

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 delN delC119 mut (trps1 Frog)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

aa806-1153 of xTRPS1. Two Cys residues in the GA…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
p076 pCS xTRPS1 delN delC320 (M)

Plasmid

#11046

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 (trps1 Frog)

Use

Mammalian, avian, and zebrafish

Mutation

aa806-952 of xTRPS1. Two Cys residues in the GAT…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
p063 pCS xTRPS1 delta N (M)

Plasmid

#11036

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 deltaN mut (trps1 Frog)

Use

Mammalian, avian, and zebrafish

Mutation

aa806-1153 of xTRPS1. Two Cys residues in the GA…

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pCI SMN1

Plasmid

#72286

Purpose

SMN1 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN1 (SMN1 Human)

Expression

Mammalian

Mutation

exons 6-7-8 including introns

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLX303-ZIM3-KRAB-dCas9

Plasmid

#154472

Purpose

Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Synthetic)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCARGO-RMCE-Xist

Plasmid

#233268

Purpose

CARGO plasmid containing ~22kb dox-inducible Xist transgene

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Insert

22kb Xist gene, including introns

Expression

Mammalian

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pR26 CAG AsiSI/MluI

Plasmid

#74286

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pIBA3-SpyCatcher-sfGFP

Plasmid

#107420

Purpose

For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

Depositor

Jack Leo

Article

Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

Insert

SpyCatcher

Tags

His tag, StrepII, and superfolder GFP

Expression

Bacterial

Promoter

Tet

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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p5E-pth2

Plasmid

#191428

Purpose

Tol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)

Depositor

Timothy Erickson

Article

Venuto et al Biol Open. 2022 Sep 26. pii: 276788. doi

Insert

pth2 regulatory region (pth2 Zebrafish)

Use

Tol2 gateway 5' entry vector

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pmRFP-LC3

Plasmid

#21075

Purpose

Mammalian expression of rat LC3 fused to mRFP

Depositor

Tamotsu Yoshimori

Article

Kimura et al Autophagy. 2007;3(5):452-60.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

mRFP

Expression

Mammalian

Mutation

First ATG is not included.

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pLX301-ZIM3-KRAB-PYL1

Plasmid

#154761

Purpose

Expresses ZIM3 KRAB domain fused to PYL1

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ZIM3 (ZIM3 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Includes ZIM3 aa 1-100

Promoter

CMV

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pR26 CAG/GFP Asc

Plasmid

#74285

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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