We narrowed to 928 results for: trna

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vHB16

Plasmid

#193455

Purpose

Expression of GFP-Cas9-NLS and gRNA in A. castellanii

Depositor

Chantal Abergel

Article

Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

Insert

tRNA-gRNA

Use

CRISPR

Promoter

U6

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMAH2-intein/mCherry

Plasmid

#71405

Purpose

Expresses suppression tRNA and mCherry inserted with a Npu DnaE intein

Depositor

Huiwang Ai

Article

Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

Insert

Npu DnaE Intein insertion in mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only
pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Expression

Bacterial

Promoter

Pbad and Ptet

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAK-EL-01

Plasmid

#125760

Purpose

Endlinker for tRNA-gRNA system when using only one guide

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK-EL-04

Plasmid

#125763

Purpose

Endlinker for tRNA-gRNA system when using only four guides

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK-EL-05

Plasmid

#125764

Purpose

Endlinker for tRNA-gRNA system when using only five guides

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Endlinker

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Pl-B

Plasmid

#79161

Purpose

Expresses the Pl-B biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Pl-B

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pULTRA-CNF

Plasmid

#48215

Purpose

highly efficient suppressor plasmid

Depositor

Peter Schultz

Article

Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

Inserts

polyspecific MJ tyrosyl synthetase
tRNA

Expression

Bacterial

Mutation

Y32L, L65V, F108W, Q109M, D158G, I159A

Promoter

proK and tacI

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-MbPylRS-4xPyOtR-ITR-GFP

Plasmid

#240124

Purpose

Express AAV2 transgene (EGFP) and MbPylRS/tRNA for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Pham et al bioRxiv 2024.07.20.604406

Inserts

AAV2-ITR-EGFP
MbPylRS
PylOtR

Use

AAV

Expression

Mammalian

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pJUMP18-pheS_NOC

Plasmid

#127031

Purpose

Basic Part NOC- Complete ORF; PheS; counter-selection marker; mutant phenylalanine tRNA synthetase.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part PheS_NOC

Use

Synthetic Biology

Expression

Bacterial

Mutation

pheS A294G promiscuous mutation

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSEVA621-AzFRS

Plasmid

#242002

Purpose

Expressed AzF tRNA synthetase for incorporation of p-azido-L-phenylalanine (pAzF)

Depositor

Wei Niu

Article

He et al ACS Synth Biol. 2022 Nov 18;11(11):3724-

Insert

AzFRS

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAMC070n1a12

Plasmid

#209746

Purpose

ColA BBa_B0014 tetAp SD8 ScTrpRS rrnB1 proB SD8 Lum1PylRS BBaB10014RNAItrpA+ J23100 SD8 MmPylRS T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

tRNA operon - ScTrpRS - Lum1PylRS - MmPylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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Strep-TGT-His

Plasmid

#206495

Purpose

Expresses E. Coli TGT with an N-terminal Strep Tag and a C-terminal His Tag

Depositor

Neal Devaraj

Article

Tota et al J Am Chem Soc. 2023 Apr 12;145(14):8099-

Insert

E. coli tRNA Guanine Transglycosylase

Tags

His and Strep

Expression

Bacterial

Promoter

T7

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_VPR_yl

Plasmid

#107677

Purpose

CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

Depositor

Ian Wheeldon

Article

Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

Inserts

Codon optimized dCas9-VPR
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Expression

Yeast

Promoter

SCR1'-tRNA and UAS1B8-TEF(136)

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-HisRS-His

Plasmid

#124111

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Histidyl-tRNA ligase (hisS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-MTF-His

Plasmid

#124133

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Methionyl-tRNA formyltransferase (fmt Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-MetRS-His

Plasmid

#124115

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Methionine--tRNA ligase (metG Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-LysRS-His

Plasmid

#124114

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Lysine-tRNA ligase (lysU Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-ProRS-His

Plasmid

#124117

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Prolyl-tRNA ligase (proS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-AspRS-His

Plasmid

#124106

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Aspartate-tRNA ligase (aspS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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