We narrowed to 16,214 results for: IGH@

1. pAAV-G12-mTGFB1

   Plasmid

   #219553

   Purpose

   mTGFB1 expression in mammlian cells. pAAV vector. Reporter plasmid.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

   Insert

   mTGFB1 (Tgfb1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   12xUAS with TATA-box minimal promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWXLd-RIEP-GpNLuc

   Plasmid

   #135935

   Purpose

   Expresses the GpNLuc LumiFluor in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   GpNLuc LumiFluor
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRN3P_T3_ABEmax_IVT

   Plasmid

   #171761

   Purpose

   Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

   Insert

   ABEmax
   

   Use

   Vector for in-vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T3 promoter

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJM251 (miniCTX1-araC-ParaBAD)

   Plasmid

   #110567

   Purpose

   Integration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   araC-ParaBAD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araC-ParaBAD

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-AKAR2-CR

   Plasmid

   #40255

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   AKAR2
   

   Use

   Tags

   His6 and Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ607-CMV-GFP-2A-Cas9

   Plasmid

   #154830

   Purpose

   GFP-2A-Cas9 (CHO codon-optimized)

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   GFP-2A-Cas9
   HygR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized (Cricetulus griseus)

   Promoter

   CMV and SV40 promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2_hMGFP-60A

   Plasmid

   #200393

   Purpose

   A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   monster GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-LacI-NLS-VP16

   Plasmid

   #103836

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   EGFP-LacI-NLS-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV2

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRN3P_T3_BE4_IVT

   Plasmid

   #171762

   Purpose

   Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

   Insert

   BE4
   

   Use

   Vector for in-vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T3 promoter

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2_RFP-60A

    Plasmid

    #200394

    Purpose

    Plasmid IVT template encoding RFP. NOTE: In the manuscript, "mCherry" was listed as the internal control for multiple fluorescence-based experiments, but it was actually RFP.

    Depositor

    Xiao Wang

    Article

    Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

    Insert

    RFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hip1R-tDimerRFP-N1

    Plasmid

    #27700

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Hip1R (Hip1r Mouse)
    

    Use

    Tags

    tDimer-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

12. mCherry-miniSOG-C1

    Plasmid

    #54801

    Purpose

    Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pJB158

    Plasmid

    #202601

    Purpose

    RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

    Depositor

    Athanasios Typas

    Article

    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

    Plasmid

    #226515

    Purpose

    Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al bioRxiv 2024.08.28.610127

    Insert

    Cox8a(4x)-SNAP-tag2-T2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFB-LIC-Bse

    Plasmid

    #26108

    Purpose

    SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

    Depositor

    Opher Gileadi

    Article

    Savitsky et al J Struct Biol. 2010 Jun 10. ():.

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    His6 - TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Oct. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKin1B

    Plasmid

    #31604

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    kinesin-1B (Kif5b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAID6

    Plasmid

    #136989

    Purpose

    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Inserts

    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO2, TDH3, TEF1, and TPI1

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMLS716

    Plasmid

    #188888

    Purpose

    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    floxed Cbr-unc-119 marker and Phsp16.41::Cre
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mc2 mNeon

    Plasmid

    #206218

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSYNCMV tAKARα

    Plasmid

    #119921

    Purpose

    AAV construct with tAKARα being expressed under the human synapsin promotor with WPRE enhancer.

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARα
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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