We narrowed to 13,677 results for: FLU;

1. pAAV-SYN-H-sDarken

   Plasmid

   #184797

   Purpose

   High affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   H-sDarken
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

   Plasmid

   #75154

   Purpose

   Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   H2B-mCerulean3-P2A-mKOFP2-CAAX
   

   Use

   Middle entry vector for multisite gateway three f…

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL5.0 shFmnl2-EGFP

   Plasmid

   #187255

   Purpose

   Lentiviral expression of shRNA targeting Fmnl2

   Depositor

   Alpha Yap

   Article

   Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

   Insert

   ShRNA for Fmnl2 (Fmnl2 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Promoter

   U6

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTDpelB-C_sfYFPTwinStrep

   Plasmid

   #45944

   Purpose

   expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Insert

   synthetic sfYFP (codon usage adapted to P.putida KT2440)
   

   Tags

   PelB export sequence and Twin-Strep-tag

   Expression

   Bacterial

   Promoter

   lacIq/Ptrc

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT2-4xUAS:P2A-mCerulean3

   Plasmid

   #127592

   Purpose

   This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mCerulean3 through P2A self-cleaving peptides.

   Depositor

   Longhou Fang

   Article

   Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

   Type

   Empty backbone

   Use

   Zebrafish expression

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCerulean3-ER-5

   Plasmid

   #55418

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   ER (CALR Human)
   

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   ER Signal Peptide

   Promoter

   CMV

   Available Since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

7. GFP-LiMETER-v1

   Plasmid

   #113933

   Purpose

   Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP

   Depositor

   Yubin Zhou

   Article

   Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

   Insert

   LOV2 and Rit polybasic domain (PB)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEX-GFP-hLC3WT

   Plasmid

   #24987

   Depositor

   Isei Tanida

   Article

   Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

   Insert

   a wild type human MAP1-LC3B fused with EGFP (MAP1LC3B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZJOM43

   Plasmid

   #133652

   Purpose

   Integration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPS4-DuDre (VPS4 Budding Yeast)
   

   Tags

   DuDre

   Expression

   Yeast

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEX-YFP-hLC3WT

    Plasmid

    #24989

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a wild type human MAP1-LC3B fused with EYFP (MAP1LC3B Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

11. PM-FRB-CFP

    Plasmid

    #67517

    Purpose

    FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    mTOR (MTOR Human)
    

    Tags

    CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…

    Expression

    Mammalian

    Mutation

    FRB domain only (residues 2019–2114)

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET22b-T5-sfGFP*-XcP4H-MH4R

    Plasmid

    #178043

    Purpose

    Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    superfolder green fluorescent protein with TAG at 151 position
    XcP4H with W179F mutation
    dihydropterine reductase from human
    pterin-4a-carbinolamine dehydratase from human
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mTagRFP-T-Gamma-Tubulin-17

    Plasmid

    #58017

    Purpose

    Localization: Centrosomes, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Gamma-Tubulin (TUBG1 Human)
    

    Tags

    mTagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. Hsp70-mplum

    Plasmid

    #45016

    Depositor

    Anna Planas

    Article

    de la Rosa et al Eur J Nucl Med Mol Imaging. 2013 Feb;40(

    Insert

    mPlum
    

    Expression

    Mammalian

    Promoter

    mouse Hspa1b

    Available Since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTDpelB-NTwinStrep

    Plasmid

    #45940

    Purpose

    use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Tags

    6xHis, PelB, and Twin-Strep

    Expression

    Bacterial

    Promoter

    Trc (lactose/IPTG inducible)

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. FAB(PX-LD)

    Plasmid

    #214426

    Purpose

    Detection of peroxisome-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FKBP-V5
    

    Tags

    CFAST-Halo and FKBP-V5-RspAN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Lyso-InPAkt

    Plasmid

    #184053

    Purpose

    Lysosome-targeted FRET-based biosensor for monitoring 3-phosphoinositide dynamics in living cells.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Lyso-InPAkt
    

    Tags

    Lysosome-associated membrane protein 1 (LAMP1).

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAM-DCA-muGFP

    Plasmid

    #166727

    Purpose

    AAV, encodes monomeric ultrastable GFP (muGFP)

    Depositor

    Daniel Scott

    Article

    Scott et al Sci Rep. 2018 Jan 12;8(1):667. doi: 10.1

    Insert

    muGFP
    

    Use

    AAV

    Promoter

    Chicken Beta Actin

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. mRuby2-Tubulin-C-18

    Plasmid

    #55915

    Purpose

    Localization: Microtubules, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Tubulin (TUBA1B Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. PB-TAC-OK+9MS-Cas9

    Plasmid

    #120354

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OK+9MS cassette
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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