We narrowed to 19,092 results for: iNOS

1. pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

   Plasmid

   #211969

   Purpose

   Express gRNA against LIN28A with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting LIN28A (LIN28A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(LIN28A_5-3)-PGKpuroBFP-W

   Plasmid

   #211968

   Purpose

   Express gRNA against LIN28A with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting LIN28A (LIN28A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO-PPO-Venus

   Plasmid

   #139505

   Purpose

   AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Has Service

   AAV8 and AAV9

   Insert

   parapinopsin
   

   Use

   AAV

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available Since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT3TS-Hf-RfxCas13d-HA

   Plasmid

   #234479

   Purpose

   Plasmid to carry out IVT of High-fidelity RfxCas13d (human codon-optimized)

   Depositor

   Miguel Angel Moreno-Mateos

   Article

   Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

   Insert

   RfxCas13d
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mutation

   Changed four alanine to valine in positions 134, …

   Promoter

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

   Plasmid

   #208959

   Purpose

   A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. px330-Axin1

   Plasmid

   #162543

   Purpose

   sgRNA targeting Axin1

   Depositor

   Amaia Lujambio

   Article

   Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

   Insert

   sgRNA targeting Axin1 (Axin1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223-PIK4CA

   Plasmid

   #23541

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PIK4CA (PI4KA Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-nEF-Con/Foff 2.0-ChRmine-oScarlet

   Plasmid

   #137161

   Purpose

   Intersectional viral expression of ChRmine-p2a-oScarlet in cells expressing Cre AND NOT Flp

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-ChRmine-p2a-oScarlet
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   oScarlet E95D

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac1-pGC-A

   Plasmid

   #186626

   Purpose

   pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

   Depositor

   Debra Hansen

   Article

   Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

   Insert

   NPR1 (NPR1 Human)
   

   Use

   Tags

   hemagglutinin (HA) signal peptide + His10-tag + T…

   Expression

   Insect

   Mutation

   deleted amino acids 1-32

   Promoter

   polyhedring

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. 161_pAAV-ProD5-CatCh-GFP-WPRE

    Plasmid

    #125981

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProD5

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. 113_pAAV-ProC21-CatCh-GFP-WPRE

    Plasmid

    #125956

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    CatCh-GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProC21

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDNA2.0 6H-TEV-KRAS G13D

    Plasmid

    #111851

    Purpose

    Expresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS G13D (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Changed Glycine 13 to Aspartic Acid

    Promoter

    T5

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(JARID2_5-4)-PGKpuroBFP-W

    Plasmid

    #211966

    Purpose

    Express gRNA against JARID2 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting JARID2 (JARID2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT3-EF1A-CCNE2

    Plasmid

    #162554

    Purpose

    Overexpression of CCNE2

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of CCNE2 (CCNE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV x EF1a_EKAREN4-ires-blast

    Plasmid

    #167829

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. miniTol2 x EF1a_EKAREN5 + PGK-puro

    Plasmid

    #167817

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV-EF1a-mCherryCXCR4-W

    Plasmid

    #200102

    Purpose

    Lentiviral vector expressing mCherry fused with CXCR4 cDNA at the C-terminus

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    silent mutations (c.180C>T, 438G>A, 495C>…

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223-PIP5K1A

    Plasmid

    #23755

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PIP5K1A (PIP5K1A Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

19. SHIP2-ΔPS

    Plasmid

    #214901

    Purpose

    expression of the truncated variants of the SHIP2 without phosphatase domain

    Depositor

    Pavel Krejčí

    Article

    Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

    Insert

    human SHIP2 delta aa 419-739 (INPPL1 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    delta 419-739aa

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. mRFP-FKBP12-5ptpase domain

    Plasmid

    #67516

    Purpose

    Recruitable human type IV 5-phosphatase domain (214-644)

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mutation

    INPP5E has C641A to destroy the C-terminal CAAX d…

    Promoter

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only