We narrowed to 10,347 results for: plasmids 101

1. SPACE-NG (pRZ5150)

   Plasmid

   #140244

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE_NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

   Promoter

   CMV

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. DDX39B_pLX307

   Plasmid

   #98331

   Purpose

   Lentiviral expression of DDX39B

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   DDX39B (DDX39B Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S296P

   Promoter

   E1Fa

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-sgAAVS1

   Plasmid

   #85802

   Purpose

   Cas9/CRISPR vector for cut at human AAVS1 gene intron1

   Depositor

   Roland Friedel

   Article

   Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

   Insert

   PPP1R12C (PPP1R12C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHL-H1-ccdB-mEF1a-RiH

   Plasmid

   #60601

   Purpose

   Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

   Depositor

   Akitsu Hotta

   Article

   Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

   Inserts

   Red Fluorescent Protein
   Hygromycin resistance gene
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. JUP_pLX307

   Plasmid

   #98347

   Purpose

   Lentiviral expression of JUP

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   JUP (JUP Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCW57.1 _3xHA-Ago2

   Plasmid

   #73538

   Purpose

   Inducible lentiviral expression of Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   Ago2 (Ago2 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLX304-YAP1 _TA

   Plasmid

   #59143

   Purpose

   YAP1 mutants that harbor a deletion of the transcriptional activation domain

   Depositor

   William Hahn

   Article

   Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   TA (transactivation) domain deleted; aa291-497

   Promoter

   CMV

   Available Since

   Oct. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pACT1:Cas9-GFP, U6:sgTK

   Plasmid

   #122852

   Purpose

   Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   Cas9-GFP
   U6-sgTK
   

   Use

   CRISPR; Cas9-gfp plasmid for genome editing of cr…

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Cryptosporidium parvum U6 and Cryptosporidium par…

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pACT1:Cas9-GFP, U6:sgUPRT

   Plasmid

   #122853

   Purpose

   Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

   Depositor

   David Sibley

   Article

   Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

   Inserts

   Cas9-GFP
   U6-sgUPRT
   

   Use

   CRISPR; Cas9-gfp plasmid for genome editing of cr…

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Cryptosporidium parvum U6 and Cryptosporidium par…

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1a-DIO-FLPo-Myc

    Plasmid

    #124641

    Purpose

    AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    FLPo
    

    Use

    AAV and Cre/Lox

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLX304-YAP1 _60-89

    Plasmid

    #59144

    Purpose

    YAP1 mutants that harbored a deletion of amino acide at 60-89 position, which disrupts binding to TEAD transcription factors

    Depositor

    William Hahn

    Article

    Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    aa60-89 deleted

    Promoter

    CMV

    Available Since

    Aug. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

    Plasmid

    #229839

    Purpose

    A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF2785

    Plasmid

    #143618

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GLIS1 (GLIS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1a-FDIO-ArchT-GFP

    Plasmid

    #124640

    Purpose

    AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    ArchT
    

    Use

    AAV; Flp/frt

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SPACE-VRQR (pRZ5137)

    Plasmid

    #140245

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE_VRQR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, VRQR mutations in SpCas9, D10A in …

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCLXSN(GFP)-HA-Slc1a5 (mouse)

    Plasmid

    #71458

    Purpose

    inducible expression fo Slc1a5 in mammalian cells

    Depositor

    Shao-Cong Sun

    Article

    Nakaya et al Immunity. 2014 May 15;40(5):692-705. doi

    Insert

    Slc1a5 (Slc1a5 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    A85P, L125F and V384A mutations compared to GenBa…

    Promoter

    CMV

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pHAGE2-TetOminiCMV-OS

    Plasmid

    #136576

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

    Plasmid

    #136660

    Purpose

    Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    ABE7.10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-lacZ

    Plasmid

    #175175

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting lacZ

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    sgRNA-lacZ
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGST-HsSyx-375-792

    Plasmid

    #179292

    Purpose

    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site

    Depositor

    Debra Hansen

    Article

    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

    Insert

    Syx
    

    Use

    Tags

    His6 + GST + TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    residues 375-792 from accession number NP_0010361…

    Promoter

    T7

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge