We narrowed to 19,203 results for: Comp;

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pHis2-Arf1 (17-181)-Q71L

Plasmid

#184808

Purpose

Bacterial expression of Arf1 (aa17-181, Q71L mutation)

Depositor

James Hurley

Article

Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

Insert

Arf1 (ARF1 Human)

Tags

His6

Expression

Bacterial

Mutation

aa 17-181 only, Q71L

Promoter

T7

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pOG-Cre

Plasmid

#233265

Purpose

Plasmid expressing Cre recombinase that is used to insert DNA CARGO into RMCE homing locus in Rosa26

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Insert

Cre recombinase

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHygromycin (GB0211)

Plasmid

#68204

Purpose

Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hygromycin resistance gene coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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F-CDK8L

Plasmid

#24762

Depositor

Joan Conaway, Ronald Conaway

Article

Conaway et al FEBS Lett. 2005 Feb 7. 579(4):904-8.

Insert

F-CDK8L (CDK19 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 14, 2010

Availability

Academic Institutions and Nonprofits only
pJRH056

Plasmid

#171637

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-tdTomato
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAGH42

Plasmid

#107249

Purpose

Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

vipp1-gfp

Use

Suicide vector for destined for double homologous…

Tags

mGFPmut3

Expression

Bacterial

Promoter

native promoter of sll0617

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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RFP-(SIM)1

Plasmid

#127094

Purpose

Bacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

mCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Tags

mCherry

Expression

Bacterial

Promoter

unknown

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

Plasmid

#79383

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Zhang 2.0 + scaffold

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA1L

Plasmid

#19457

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1L (HSPA1L Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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A3Bi-Cas9n-UGI

Plasmid

#119135

Purpose

Base editor made from full-length APOBEC3B with an L1 intron to prevent self-restriction

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3B L1 intron-Cas9 nickase-UGI

Use

CRISPR

Mutation

L1 intron insertion

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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p6899 PHAGE-P CMVT N-HA-hE6AP II C843A

Plasmid

#37604

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP II C843A (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C843A

Promoter

CMVt

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pFA-DAB2

Plasmid

#162690

Purpose

Expresses DabAB2 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB2 inorganic carbon transport complex

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER IKKalpha

Plasmid

#26208

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK alpha (CHUK Human)

Use

RNAi

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pFlox_N-p300_BSD_FKBPF36V_EYFP

Plasmid

#163885

Purpose

Acute Depletion P300-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

P300 (Ep300 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Vh-HA-3xFKBP

Plasmid

#20222

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFKBP (FKBP1A Human)

Tags

HA and Vh

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
Flag Nprl2 pLJM1

Plasmid

#46338

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl2 (NPRL2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pscALPS-SIV-CPZ-TAN3 vpr

Plasmid

#115833

Purpose

Encodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVCPZ-TAN3 Vpr (DQ374658)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBS mouse c-Myc

Plasmid

#14972

Depositor

Joan Massague

Article

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (Myc Mouse)

Expression

Bacterial

Mutation

Nucleotides 2578-3294

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pIC292

Plasmid

#51405

Purpose

Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Article

Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

Insert

Ska2 IMAGE 4685972 (SKA2 Human)

Use

Retroviral

Tags

EGFP, S tag, and TEV protease site

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-LckAPEX-P2A-EGFP

Plasmid

#182826

Purpose

Cre-dependent expression of membrane localized APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

LckAPEX-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Promoter

EF1a

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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