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pGEX Nsp1-SH2

Plasmid

#46459

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nsp1 (SH2D3A Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 3-119

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Slnk-SH2

Plasmid

#46502

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slnk (Sh2d6 Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 199-323

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Socs7-SH2

Plasmid

#46509

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Socs7 (SOCS4 Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 280-386

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pAG415-ADH1p-HA-rap1-K9R

Plasmid

#84531

Purpose

This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

HA

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

ADH1

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3NR

Plasmid

#84533

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,117,118)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K3CR

Plasmid

#84534

Purpose

This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(462,463,651)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K9R

Plasmid

#84535

Purpose

This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K651R

Plasmid

#84536

Purpose

This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Tags

EGFP

Expression

Yeast

Mutation

K651R

Promoter

GPD

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
CSII-IDR2-AGMpygTRIM5-FOS

Plasmid

#79064

Purpose

Express African green monkey pygerythrus TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(African green monkey pygerythrus)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rab11 CA

Plasmid

#53472

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Tags

YFP

Expression

Insect

Mutation

Q70L

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab32 DN

Plasmid

#37739

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T33N (dominant negative)

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pMT3-RhoGC E254D

Plasmid

#100033

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC E254D

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

E254D

Promoter

Adenovirus major late promoter

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)

Plasmid

#60245

Purpose

Express the RBD of mouse MSI1 in bacterial cells for protein purification

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

contains aa7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-192)R61E

Plasmid

#60353

Purpose

R61E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R61E; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab32 CA

Plasmid

#37740

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q79L (constitutive active)

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pWzl_neo_DEST_flag_BFP

Plasmid

#45302

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

BFP

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pTJK281 (miR-513a-5p mut OE in pWCC52)

Plasmid

#254386

Purpose

Express human miR-513a-5p mutant

Depositor

Curt Civin

Article

Kim et al FEBS Open Bio. 2026 Mar 8. doi: 10.1002/

Insert

miR-513a-5p mutant (containing 2 base mutations in mature miR-513a-5p sequence) (MIR513A1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pTJK282 (miR-513a-3p mut OE in pWCC52)

Plasmid

#254387

Purpose

Express human miR-513a-3p mutant

Depositor

Curt Civin

Article

Kim et al FEBS Open Bio. 2026 Mar 8. doi: 10.1002/

Insert

miR-513a-3p mutant (containing 2 base mutations in mature miR-513a-3p sequence) (MIR513A1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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Modified pYTK075

Plasmid

#254020

Purpose

A part plasmid designed to use the yeast selective marker (LEU2 gene) as a Level 1 TU fragment.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

LEU2

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)--FLEx(loxP)-splitTVA:HA

Plasmid

#248665

Purpose

Helper virus for monosynaptic tracing; Expresses TVA and an HA tag in Cre-expressing cells under the CAG promoter. To be co-injected with Rabies Glycoprotein (G) viruses.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

TVA

Use

AAV

Tags

HA

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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