We narrowed to 15,990 results for: nes

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AAV-hRedOpsin-TGFB3

Plasmid

#164429

Purpose

AAV plasmid expressing TGFB3 in photoreceptors

Depositor

Connie Cepko

Article

Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

Insert

Tgfb3 (Tgfb3 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF RBP-FL P53

Plasmid

#174042

Purpose

Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

Depositor

Stewart Loh

Article

Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

Insert

P53 gene (TP53 Human)

Tags

Thermoanaerobacter Tencongenesis ribose binding p…

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-2_dCrk

Plasmid

#131146

Purpose

Expresses GST::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

GST

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCIneoLuc-PP1A

Plasmid

#128348

Purpose

Vector for LUMIER assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

PP1A (PPP1CA Human)

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST47-CYFIP2.GFP

Plasmid

#109140

Purpose

GFP-tagged CYFIP2 expression construct

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP2 (CYFIP2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-hA1-BE3

Plasmid

#113418

Purpose

Expresses hA1-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA1-BE3 (APOBEC1 S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean3-PCNA-19-SV40NLS-4

Plasmid

#55437

Purpose

Localization: Nucleus, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

PCNA-19 SV40NLS (PCNA Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pME-hygro-hPGAP3-3HA

Plasmid

#50373

Purpose

Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)

Tags

3x HA

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pCR4-Dact1(1250-1601)

Plasmid

#52046

Purpose

To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.

Depositor

Ben Cheyette

Article

Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.

Insert

Dact1(1250-1601) (Dact1 Mouse)

Expression

Bacterial

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-1224

Plasmid

#26337

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1224 (Mir1224 Mouse)

Expression

Mammalian

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only
MALT1-DD (2G7R)

Plasmid

#25613

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

MALT1-DD (MALT1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains Death Domain

Available Since

Aug. 31, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pIS1 IMP-1 rc

Plasmid

#21643

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCSP1-mp53AS

Plasmid

#20896

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

p53AS (Trp53 Mouse)

Expression

Mammalian

Mutation

5' natural EcoRV and SacI restriction sites …

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Hygro- TRF2 deltaM

Plasmid

#18012

Depositor

Titia de Lange

Article

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Deleted Myb Domain (aa454-500)

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
CS2 luc Utrn

Plasmid

#13500

Depositor

Stephen Tapscott

Article

Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

Insert

Utrophin (Utrn Mouse)

Use

Luciferase

Mutation

Contains 5 copies of nt 10657-10693 of NM_011682.

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pPE3-pPuro

Plasmid

#215722

Purpose

Cloning vector for Uni-Vector prime editing system

Depositor

Kou-Juey Wu

Article

Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

Insert

PE2

Use

Unspecified

Promoter

EFS promoter

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Puro-PNPT1 S484A

Plasmid

#223316

Purpose

Lentiviral vector expressing human PNPT1 S484A variant

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed serine 484 to alanine

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

Plasmid

#224497

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax

Use

Gateway Cloning

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-Qa1 SCT-T2A-GFP

Plasmid

#205453

Purpose

lentiviral plasmid for expression of mouse Qa1 SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

Qa1 SCT (H2-T23 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-TYK2K930R-VSV

Plasmid

#139346

Purpose

Expression of kinase-dead TYK2-K930R protein

Depositor

Sandra Pellegrini

Article

Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-K930R

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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