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Plasmid#144615PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by
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TFORF3138
Plasmid#144614PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
vYM140_PB-pEFa1-iaaM(P.s)-Hygro
Plasmid#160043PurposeExpressing iaaM for converting L-Trp to IAMDepositorAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2
Plasmid#177919PurposeExpresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-F30-Broccoli-Squash-SAM-sensor 4-2
ExpressionMammalianPromoterU6+27Available sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0352 HvsCas7-11 with C-term Nes mammalian expression
Plasmid#172516PurposeEncodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cellsDepositorInsertC-term Nes HvsCas7-11 mammalian expression
ExpressionMammalianAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM149
Plasmid#133646PurposeIntegration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).DepositorAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEf1a OsTIR ires NEO pA T2BH
Plasmid#127910PurposeTransposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoterDepositorInsertOsTIR
UseTransposonAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP L202 gRNA
Plasmid#119132PurposegRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP L202 gRNA
UseCRISPRMutationNonePromoterU6Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd3
Plasmid#98298PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
ExpressionYeastAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ155
Plasmid#69306PurposeGateway entry vector with AteCas9D10ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAteCas9D10A (Arabidopsis codon optimized)
UseCRISPRMutationD10A mutation; NLS fusionAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINEneo-3C-GFP
Plasmid#53534PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP
TagsGFP-His8ExpressionBacterial, Insect, and Mamm…Available sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAP Z alpha 1 beta 1 CP
Plasmid#13451DepositorAvailable sinceDec. 22, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET His6 MBP TEV HDAC8_374
Plasmid#122174PurposeExpresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tagDepositorInsertHis6 MBP TEV HDAC8_374 (HDAC8 Human)
Tagshexahistidine tag and tobacco etch virus protease…ExpressionBacterialMutationResidues Met1–Pro7 and H375-V377 have been delete…PromoterT7Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB_EF1a-DjCas13d-msfGFP-Blast
Plasmid#226008PurposeDjCas13d protein for RNA targeting in piggyBac backboneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDjCas13d RNA-targeting nuclease
UseCRISPRTagsmsfGFPExpressionMammalianAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-MDM4
Plasmid#195140PurposeTet-ON 3rd generation expression vector with inducible expression of MDM4 and a puromycin selection cassetteDepositorAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-HaloTag-Puromycin
Plasmid#167208PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsHaloTagExpressionMammalianAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
elavl3:SomaGCaMP7f
Plasmid#158760PurposeCell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSomaGCaMP7
UseSynthetic BiologyPromoterHuC promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLC-ZsGreen-P2A-Blast
Plasmid#123322PurposeLentiviral vector for expression of ZsGreen-P2A-Blast cassette from a CMV promoterDepositorInsertZsGreen
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMM310-Myc
Plasmid#80053PurposeExpression of a Myc tagged version of the BlaM-Vpr fusion proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMyc-BlaM-Vpr
TagsMycExpressionMammalianMutationN-terminal Myc tag added to construct pMM310Available sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMU-MITr
Plasmid#61198PurposeFluorescent reporter for mitochondria ScCOX4sp-mCherry expressed under AtUBQ10 promoterDepositorInsertScCOX4sp-mCherry
TagsmCherryExpressionPlantPromoterAtUBQ10Available sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only