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pET-SOMA-T679A

Plasmid

#118936

Purpose

Used to express the T679A mutant form of the SOMA FRET sensor in E. coli.

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana with T679A Mutation

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-PDAE

Plasmid

#110180

Purpose

Expresses propanediol dehydratase activating enzyme in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase Activating Enzyme

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD

Plasmid

#110168

Purpose

Expresses propanediol dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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p1G108CFa

Plasmid

#85089

Purpose

Expresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

The G108C variant of PCNA1 fused to an FAD domain of P450 BM3

Tags

His6 tag

Expression

Bacterial

Mutation

G108C in PCNA1, A74G/C773S/C810S in FAD domain of…

Promoter

T7 promoter

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET24a_UDP_Xase_E162

Plasmid

#73160

Purpose

Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E162A

Depositor

Sandra Gabelli

Article

Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

Insert

pET24a

Expression

Bacterial

Mutation

change glutamate 162 to alanine

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF3-V5-His6

Plasmid

#67412

Purpose

Expression of hArf3 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF3 (ARF3 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF4-V5-His6

Plasmid

#67413

Purpose

Expression of hArf4 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF5-V5-His6

Plasmid

#67414

Purpose

Expression of hArf5 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL2-V5-His6

Plasmid

#67417

Purpose

Expression of hArl2 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL8A-V5-His6

Plasmid

#67424

Purpose

Expression of hArl8 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL4D

Plasmid

#67384

Purpose

Expression of native hArl4D in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL11

Plasmid

#67386

Purpose

Expression of native hArl11 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Expression

Bacterial

Mutation

bp 261 G to A; silent mutation; bp 442 T to C; aa…

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pGS1613

Plasmid

#63789

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Rai et al Mol Biotechnol. 2014 Jun 27.

Insert

tDimer2-Strep-tag II followed by HIS5

Use

E. coli cloning vector

Tags

tDimer2-Strep-tag II

Promoter

none

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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23SrRNA.RAV12(wt).2508-2580.GAAAC.G2553U

Plasmid

#26191

Depositor

David McKay

Article

Hardin et al J Mol Biol. 2010 Sep 17;402(2):412-27. d

Insert

23S ribosomal RNA fragment (modified) with G2553U mutation

Expression

Bacterial

Mutation

fragment 2508-2580 of E. coli rRNA, with nucleoti…

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only

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RGB-S reporter

Plasmid

#207841

Purpose

A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

Depositor

Christof Niemeyer

Article

Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

Inserts

RpoH sensing construct
SOS sensing construct
RpoS sensing construct

Expression

Bacterial

Promoter

PosmY, PsulA, and grpE

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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PACT wild type

Plasmid

#251697

Purpose

PRKRA protein expression construct in E. coli

Depositor

Sun Hur

Article

Ahmad et al Nat Commun. 2025 Apr 8;16(1):3325. doi:

Insert

PRKRA (PRKRA Human)

Expression

Bacterial

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPe_PepTSo

Plasmid

#58334

Purpose

Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

So Iwata, Simon Newstead

Article

Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

Insert

PepTso

Tags

GFP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
SepRS(2)/pSertRNA(B4)/EF-Sep

Plasmid

#173897

Purpose

The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

Depositor

Jason W Chin

Article

Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

Inserts

SepRS(2)
pSertRNA
EF-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAIDA_TEV_fap2EC

Plasmid

#246866

Purpose

Expression of Fusobacterium nucleatum ATCC23726 Fap2 extracellular domain (42-3271) with histag and TEV site in fusion with E. coli autotransporter AIDA

Depositor

Daniel Roderer

Article

Schopf et al Nat Commun. 2025 Aug 29;16(1):8104. doi:

Insert

F. nucleatum ATCC23726 Fap2 (extracellular domain, res. 42–3271)

Tags

8x histidine, AIDA autotransporter, AIDA signal s…

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-HNRNPA1LCD

Plasmid

#204405

Purpose

E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

HNRNPA1 (186-320) (HNRNPA1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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