We narrowed to 9,182 results for: Pol

1. pDB007ns2a

   Plasmid

   #99214

   Purpose

   Accessory plasmid for PACE of PylRS variants; negative selection

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
   

   Mutation

   amber codons at positions 12 and 203 of T7 RNA po…

   Promoter

   Ppsp, PproK, and Ptet

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP6-sgRNA-scaffold

   Plasmid

   #47912

   Purpose

   Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection

   Depositor

   Paul Sternberg

   Article

   Chiu et al Genetics. 2013 Aug 26.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   sgRNA 3' end

   Expression

   Worm

   Promoter

   SP6

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCbeta EV (PKA catalytic subunit Cbeta)

   Plasmid

   #15311

   Depositor

   Stanley McKnight

   Article

   Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

   Insert

   PRKACB (Prkacb Mouse)
   

   Expression

   Mammalian

   Available Since

   July 6, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pDB023f1

   Plasmid

   #99218

   Purpose

   Complementary plasmid for aaRS PACE; amber codons in T7 RNAP

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Ppsp [SD4] T7RNAP(S12*,S203*)
   

   Expression

   Bacterial

   Mutation

   amber codons at positions 12 and 203 of T7 RNA po…

   Promoter

   Ppsp

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. ERAP1

   Plasmid

   #39174

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ERAP1 (ERAP1 Human)
   

   Use

   Baculovirus expression

   Tags

   TEV-His10-Flag

   Promoter

   polyhedrin

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

6. LBJJ382

   Plasmid

   #117518

   Purpose

   Also known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminator

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU6-26

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOCC82

   Plasmid

   #118862

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant protein

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-stop-HindIII cassette
   

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MXS_bGpA

   Plasmid

   #62424

   Purpose

   MXS_chaining vector with bGpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   beta-Globin poly A
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRset-OppA

   Plasmid

   #207869

   Purpose

   Bacterial expression of poly-his-tagged L. lactic OppA

   Depositor

   Mathew Tantama

   Article

   Norcross et al ACS Omega. 2019 Feb 28;4(2):2812-2822. d

   Insert

   OppA
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-T3-eSpCAS9-NG-pA

    Plasmid

    #131466

    Purpose

    Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

    Insert

    Codon optimized eSpCas9-NG
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. hPDI1-18-508_C400S-pFLAG-CMV1

    Plasmid

    #31384

    Depositor

    David Ron

    Article

    Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

    Insert

    hPDI1 (P4HB Human)
    

    Tags

    FLAG M1

    Expression

    Mammalian

    Mutation

    C400S

    Available Since

    May 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBACgus N-His/GST/LRRK2 1867-2186

    Plasmid

    #40403

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    GST-Tev and HisTev

    Mutation

    WT aa1867-2186

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pSB_init-Sb44

    Plasmid

    #159421

    Purpose

    Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    Sb44
    

    Tags

    c-Myc and poly-His

    Expression

    Bacterial

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE80L TriCatcher-ELP

    Plasmid

    #112630

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-ELP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-T3-SpCAS9-NG-pA

    Plasmid

    #131465

    Purpose

    Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

    Insert

    Codon optimized SpCas9-NG
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15bLCFPT7

    Plasmid

    #53545

    Purpose

    Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant

    Depositor

    Lingchong You

    Article

    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

    Insert

    T7 RNAP
    

    Expression

    Bacterial

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393

    Plasmid

    #216382

    Purpose

    Baculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Tetraspanin-12 (TSPAN12 Human)
    

    Use

    Baculovirus

    Tags

    6xHis-3C-splitGFP11

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4092709

    Plasmid

    #153859

    Purpose

    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Mutation

    Nearly native encoding;handful SNPs as watermarks…

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pBac(3xP3-gTc’v; Tc-hsp68:Cre)

    Plasmid

    #119063

    Purpose

    Expression of Cre recombinase in Tribolium castaneum

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2019 Jan 4;146(1). pii: dev

    Inserts

    pBacL
    Tc-hsp68 Promoter
    Cre recombinase
    Tc-hsp68 3'UTR
    SV40polyA
    Tc-vermilion
    3xP3 TATA
    pBacR
    

    Expression

    Insect

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. HOXA9-MSCV-full-GFP

    Plasmid

    #20977

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2008 Jul . 28(14):4609-19

    Insert

    HOXA9 (Hoxa9 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Full length cDNA with ~1.1kb 3'UTR with 2 pu…

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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