We narrowed to 17,098 results for: IGH@

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FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Plasmid

#58442

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-His6

Plasmid

#182386

Purpose

Bacterial expression of Vesicle Nucleating peptide

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTC217

Plasmid

#70018

Purpose

Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (Cas9/sgRNA) + Donor + GVR

Use

CRISPR

Expression

Plant

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::ZeoR-bGHpA

Plasmid

#62442

Purpose

MXS_chaining vector with PGK::ZeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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mc2 puro

Plasmid

#206215

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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HcKCR1_mCherry_pcDNA3.1

Plasmid

#177336

Purpose

Expresses KCR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR1

Tags

mCherry

Expression

Mammalian

Mutation

mammalian codon-adapted

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTPH418b

Plasmid

#185725

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C)

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTPH412

Plasmid

#185724

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-gp41-8N

Use

CRISPR

Expression

Bacterial

Mutation

ecTadA(8e) R26G

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-XRCC4

Plasmid

#46959

Purpose

Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

XRCC4 (XRCC4 Human)

Tags

GFP-2xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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CT752

Plasmid

#233658

Purpose

Flp GFP Reporter; FRT-STOP-FRT-GFP

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

FRT-STOP-FRT-GFP

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT769

Plasmid

#233661

Purpose

Split Flp on same plasmid; PhyA-Flp-397-Flp-396-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

PhyA-Flp-397-Flp-396-FHY1

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYNCMV tAKARα

Plasmid

#119921

Purpose

AAV construct with tAKARα being expressed under the human synapsin promotor with WPRE enhancer.

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARα

Use

AAV

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-CAG-mStayGold

Plasmid

#241387

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CAG promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pFUGW-hGtACR2-EYFP

Plasmid

#67877

Purpose

Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

Depositor

John Spudich

Article

Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

Insert

hGtACR2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

UbC

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCDB179-ThermoPETase

Plasmid

#218698

Purpose

Encodes codon optimized ThermoPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

ThermoPETase

Tags

10xHis

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTPH405c

Plasmid

#185726

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C) single PAM linker

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAJM.771

Plasmid

#108534

Purpose

NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

NahRAM + PSalTTC-YFP

Expression

Bacterial

Mutation

NahR: I25V P72H V118A R248C

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMAH-POLY

Plasmid

#64915

Purpose

Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

Depositor

Huiwang Ai

Article

Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

Inserts

Polyspecific aminoacyl-tRNA Synthetase
Amber Suppression tRNA

Tags

None

Expression

Mammalian

Promoter

CMV and U6/H1

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pBiFC-bJunVN155(I152L)

Plasmid

#27098

Depositor

Chang-Deng Hu

Article

Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

Insert

bJunVN155(I152L) (Jun and Aequorea Victoria, Rat)

Tags

Myc

Expression

Mammalian

Mutation

I152L in Venus(1-154)

Available Since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pMDH

Plasmid

#133129

Purpose

A recombinant plasmid expressing mdh (malate dehydrogenase) gene

Depositor

Gregg Beckham

Article

Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

Insert

malate dehydrogenase gene

Expression

Bacterial

Promoter

malate dehydrogenase promoter

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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