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LENTICRISPR-TKT_sgRNA1

Plasmid

#201624

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TKT (TKT Human)

Use

CRISPR and Lentiviral

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-tev-std

Plasmid

#109423

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope

Tags

BAP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pENeGFP PCNAL2 pc653

Plasmid

#167564

Purpose

The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

Depositor

Cristina Cardoso

Article

Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-KPNA2

Plasmid

#192306

Purpose

Gateway entry vector for an inducible 3xFLAG-KPNA2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA2 (KPNA2 Human)

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Pou5f1

Plasmid

#174872

Purpose

CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)

Use

CRISPR

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_r5-HT1.0

Plasmid

#208725

Purpose

Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Red fluorescent 5-HT sensor GRAB_r5-HT1.0

Use

AAV

Promoter

EF1a

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPN062

Plasmid

#91595

Purpose

Express sgRNA targeting human DFNA5

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DFNA5 (GSDME Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN063

Plasmid

#91596

Purpose

Express sgRNA targeting human DFNA5

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DFNA5 (GSDME Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Ago2_1

Plasmid

#73529

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Ago2_2

Plasmid

#73530

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
ESR1-Tol2

Plasmid

#113382

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Tol2 reporter

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only
ATP9A-Tol2

Plasmid

#113385

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ATP9A-enhancer (ATP9A Human)

Use

Tol2 reporter

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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ALARM NMR

Plasmid

#90209

Purpose

Expresses the human La antigen for use in ALARM NMR

Depositor

Michael Walters

Article

Dahlin et al Nat Commun. 2017 Nov 15;8(1):1527. doi:

Insert

La antigen (SSB Human)

Tags

6xHis

Expression

Bacterial

Mutation

contains amino acids 100-324

Promoter

T7 promoter

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeAQ482A

Plasmid

#128945

Purpose

expresses Q482A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-MCU-S259A

Plasmid

#31728

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Mutation

Changed S259 to Alanine

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pSpCas9-Puro HLAG-2A-sgRNA

Plasmid

#182551

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Article

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2A-sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCX4-cMyc

Plasmid

#36119

Depositor

Steven Dowdy

Article

Israel et al Nature. 2012 Jan 25;482(7384):216-20. do

Insert

cMyc (MYC Human)

Use

Retroviral

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only
QSOX1-Tol2

Plasmid

#113383

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

QSOX1-enhancer (QSOX1 Human)

Use

Tol2 reporter

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
AAVS1-Puro Xlone SOX17

Plasmid

#179838

Purpose

Doxycycline-inducible expression of human SOX17

Depositor

Xiaoping Bao

Article

Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

Insert

SOX17 (SOX17 Human)

Use

AAV, CRISPR, Synthetic Biology, and TALEN ; Donor…

Expression

Mammalian

Promoter

TRE3GS

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG665 MAP4-pFAST

Plasmid

#172865

Purpose

Expresses MAP4-pFAST in mammalian cells (microtubule labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

MAP4-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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