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Mito-mEosEM-E

Plasmid

#226335

Purpose

Photoswitchable fluorescent protein for correlative light-electron microscopy; mitochondria-targeted mEosEM-E for mammalian expression

Depositor

Pingyong Xu

Article

Peng et al Cells. 2022 Mar 23;11(7):1077. doi: 10.3

Insert

COX8A (COX8A Human)

Tags

mEosEM-E

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37a, 46]

Plasmid

#26569

Depositor

Diane Lipscombe

Article

Bell et al Neuron. 2004 Jan 8. 41(1):127-38.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 24a. Contains …

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO V5 DNAJB7

Plasmid

#19530

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily B, member 7 (DNAJB7 Human)

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-JNKAR

Plasmid

#181857

Purpose

Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-JNKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AktAR

Plasmid

#181856

Purpose

Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AktAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TREK-2

Plasmid

#224769

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]

Plasmid

#26571

Depositor

Diane Lipscombe

Article

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 31a Contains e…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
LLP787_SnoopCatcher-EZH2-FL-CO

Plasmid

#211780

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-DNMT3A

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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p164 Dync1i1.B

Plasmid

#26435

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform B (Dync1i1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pCosSAD1AprR

Plasmid

#190786

Purpose

An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination

Depositor

Jan Kormanec

Article

Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

Inserts

upstream region of the aur1 cluster for auricin
ampramycin resistance cassette
downstream region of the aur1 cluster for auricin

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Q769gp120-8his

Plasmid

#100929

Purpose

Mammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-NUbo-GFP-His6

Plasmid

#212790

Purpose

Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-GFP-His6

Use

Integrative vector

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMA7

Plasmid

#79967

Purpose

TM-MAGE strain

Depositor

Morten Sommer

Article

Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

Inserts

Lambda Red recombinase beta subunit
DNA adenine methylase

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Dynactin-4/p62-3XHA

Plasmid

#31058

Depositor

Vann Bennett

Article

Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

Insert

Dynactin-4 (DCTN4 Human)

Tags

3XHA

Expression

Mammalian

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-alpha4 wt (Hygro)

Plasmid

#80017

Purpose

Mammalian expression of human integrin alpha4, Hygro selection

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]

Plasmid

#26570

Depositor

Diane Lipscombe

Article

Lin et al Neuron. 1997 Jan . 18(1):153-66.

Insert

calcium channel alpha-1B subunit (Cacna1b Rat)

Tags

6XHis and V5

Expression

Mammalian

Mutation

Deletion of exons a10, 18a, and 24a. Contains …

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pBbB8k-csg-NbMS2

Plasmid

#171701

Purpose

Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.

Depositor

Neel Joshi

Article

Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1

Insert

csgBACEFG, with nanobody fused to CsgA

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGG-TurboID-GFP-PTS1

Plasmid

#209406

Purpose

Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID-PTS1

Tags

GFP

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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p199 Dync1i2.C (Ex1a)

Plasmid

#26449

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
pEGFPm-DAD M1041A

Plasmid

#25411

Depositor

Arthur Alberts

Article

Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

Insert

Diap3 (Diaph3 Mouse)

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

Alanine substitution at critical residue in DAD t…

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only