We narrowed to 10,347 results for: plasmids 101

1. B270 + SMARCAL1 sgSTOP

   Plasmid

   #100717

   Purpose

   B270 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting SMARCAL1 (cloned using BbsI) and ATP1A1 (SMARCAL1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRetroX-TRE3G-PLOD2_PGK-GpNLuc

   Plasmid

   #136457

   Purpose

   Expresses Tet/Dox-inducible human PLOD2 in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

   Insert

   TRE3G::PLOD2-T2A-Hygromycin (PLOD2 Human)
   

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBK1303-AAV-EFSNC-dCjCas9-MECP2(204-310)

   Plasmid

   #223148

   Purpose

   Expression of truncated MECP2 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310

   Promoter

   EF1a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-DIO {hCAR}off-{DTR-GFP}on-W3SL

   Plasmid

   #111396

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation), and W3SL cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   hCAR (CXADR Human)
   DTR-EGFP
   

   Use

   AAV

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

   Plasmid

   #118412

   Purpose

   Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.

   Depositor

   Nicole Paulk

   Article

   Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1

   Insert

   Firefly luciferase
   

   Use

   AAV, Luciferase, and Synthetic Biology

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCW57.1 _3xHA-Ago2_RNAmut

   Plasmid

   #73539

   Purpose

   Inducible lentiviral expression of Ago2_RNAmut

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   Ago2 (Ago2 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Small RNA binding mutant PAZ domain Y311A and F31…

   Promoter

   TRE promoter, Tet ON

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-STAT3

   Plasmid

   #195575

   Purpose

   Expresses STAT3

   Depositor

   Kevin Park

   Article

   Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

   Insert

   Signal transducer and activator of transcription 3 (Stat3 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer and promoter

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCW57.1 _3xHA-Ago2_CATmut

   Plasmid

   #73540

   Purpose

   Inducible lentiviral expression of Ago2_CATmut

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   Ago2 (Ago2 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Catalytic mutant PIWI domain D670A

   Promoter

   TRE promoter, Tet ON

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV2_hSyn_Phobos_CA_Citrine

   Plasmid

   #98218

   Purpose

   Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Phobos_C128A gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…

   Promoter

   human synapsin

   Available Since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP

    Plasmid

    #50834

    Purpose

    An AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.

    Depositor

    Brandon Harvey

    Article

    McDevitt et al Cell Rep. 2014 Sep 25;8(6):1857-1869. do

    Insert

    hChR2 (H134R)
    

    Use

    AAV and Cre/Lox

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    EF1a

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHAGE2-TetOminiCMV-SM

    Plasmid

    #136578

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-E2A-cMyc (Sox2, Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CNTF-HA

    Plasmid

    #195517

    Purpose

    Expresses HA-tagged CNTF in mammalian cells

    Depositor

    Kevin Park

    Article

    Ribeiro et al Exp Neurol. 2022 Sep;355:114147. doi: 10

    Insert

    Ciliary neurotrophic factor (CNTF Human)
    

    Use

    AAV

    Tags

    HA-tag and NGF signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    UbC promoter with beta-globin intron

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MSCV-NSD3-short del(280-342)

    Plasmid

    #72550

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 280-342 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    280-342 aa deletion

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MSCV-NSD3-short del(384-645)

    Plasmid

    #72549

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 384-645 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    384-645 aa deletion

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DYNLT3_pLX307

    Plasmid

    #98329

    Purpose

    Lentiviral expression of DYNLT3

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    DYNLT3 (DYNLT3 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MSCV-NSD3-short del(100-263)

    Plasmid

    #72548

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 100-263 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3xFlag and SV40 NLS

    Expression

    Mammalian

    Mutation

    100-263 aa deletion

    Promoter

    LTR

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PB_rtTA_BsmB-HNRNPK_sgRNA_4

    Plasmid

    #242899

    Purpose

    PiggyBac cargo vector with HNRNPK sgRNA 4 for dox-inducible knockdown

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

    Insert

    HNRNPK (Hnrnpk Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB_rtTA_BsmB-HNRNPK_sgRNA_1

    Plasmid

    #242897

    Purpose

    PiggyBac cargo vector with HNRNPK sgRNA 1 for dox-inducible knockdown

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

    Insert

    HNRNPK (Hnrnpk Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB_rtTA_BsmB-HNRNPK_sgRNA_3

    Plasmid

    #242898

    Purpose

    PiggyBac cargo vector with HNRNPK sgRNA 3 for dox-inducible knockdown

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al Mol Cell. 2019 Aug 8;75(3):523-537.e10.

    Insert

    HNRNPK (Hnrnpk Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV_Ckm_F9

    Plasmid

    #226140

    Purpose

    AAV construct for HITI insert with Ckm gRNA and hF9 transgene

    Depositor

    Christopher Nelson

    Article

    Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

    Inserts

    F9 (F9 Human)
    Ckm gRNA (Ckm Mouse)
    EGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge